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Freezing

BAMBANKER™ serum-free cell freezing media is specially formulated to minimise cell damage during the freezing process and improve survival rates.

Cryogenic Storage

Storing banks of valuable cell lines for future use and reference is essential for many researchers and the technique of cryopreservation is a mainstay for numerous labs.  By cooling cells to sub-zero temperatures biological activity is effectively stopped.  But ultra low temperature storage is not without risk and cells can incur freezing injuries that compromise viability after thawing.

Freezing Cells

Cells can be directly damaged by the formation of ice crystals.  When cooled slowly water migrates out of the cells forming ice in the extracellular spaces, too much ice can cause mechanical damage to the cell membrane and crush the cell.  Similarly, intracellular ice formation can disrupt organelles and cause lethal damage.  As water freezes, solutes such as salt are excluded and become concentrated in the remaining liquid which can prove toxic.  The migration of water can also result in cellular dehydration.

Cryoprotectant Solutions

Adaptive mechanisms to protect organisms exposed to very low temperatures can be observed in the natural world.  Microscopic multicellular organisms called ‘Water-bears’ (Tardigrada) survive freezing by replacing most of their internal water with the sugar trehalose, preventing it from crystallisation that would damage cell membranes. North American wood frogs have also evolved freezing survival strategies; they accumulate urea in tissues in preparation for overwintering and increase release of glucose from the liver to prevent internal ice formation.  Using solute solutions as cryoprotectants can significantly reduce the risks of freezing injury.

Cell Freezing Media

The unique, patented formulation of BAMBANKERTM serum-free cell freezing medium has been optimised to give excellent cell recovery.  Developed by Lymphotec Inc to preserve even delicate cells during medium and long term ultra low temperature storage, BAMBANKER has been tested with established cell lines, primaries and stem cells.  Both human and animal cells have been successfully preserved, demonstrating excellent survival rates.

 

Cryogenic preservation of cell cultures is a popular way to maintain back up cultures and reserves of cell lines.

Bambanker is ready-to use, no dilution or addition of DMSO/glycerol are required. Simply harvest cells during the logarithmic growth phase, aspirate media and resuspend in BAMBANKER before freezing.  

There is no need for programmable freezers or step-wise temperature reduction just freeze cells directly at -80C.  Frozen cells can subsequently be transferred to liquid nitrogen storage facilities if desired. 

A stringent quality assurance programme ensures protection for your precious cell cultures with sterility, endotoxin, mycoplasma, fungi and bacteria testing on every batch. 

Available in two pack sizes, a single 120ml bottle for high volume users storing large batches of cells or 5x20ml bottles. 

Ideal for dedicated use with specific cell lines or culture areas to minimise cross contamination of cultures and culture facilities.

  • Ready to use
  • Freeze and store at -80C
  • Stringent QA including mycoplasma testing
  • Serum-free
  • Choice of pack sizes


HeLaS3 Cells Cryopreservation Data

Before freezing

BAMBANKER before Freezing

3 days after thawing

BAMBANKER after Thawing

  • Bambanker Cell Freezing Media Flyer

    • PDF size: 9.89KB
    • Uploaded on: 21/09/2015

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  • BAMBANKER Cell Survival Data

    • PDF size: 7.63KB
    • Uploaded on: 18/09/2015

    A comparison of BAMBANKER against other commercially available products for various cell lines .

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User Review on BioCompare

Endothelial Progenitor cells

Bone Marrow T Cells

Adipose stem cells

HS-RMS-2 (rhabdomyosarcoma cell line)

Primary calvarial osteoblasts

More Literature References Featuring BAMBANKER

1. Takata Y. et al.: Generation of iPS cells using a BacMam multigene expression system. Cell Struct Funct., 36(2): 209-222 (2011)

2. Thuy BH, et al.: Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction, 141: 67-77 (2011).

3. Sato D., et al.,: Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients., Nephrology Dialysis Transplantation, (2011)

4. Huang MS, et al., Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp. Journal of Dental Sciences, 6(1): 48-52 (2011))

5. Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics, 140 (6): 799-805 (2011)

6. Oshima N., et al., Optimized method for culturing outgrowth endothelial progenitor cells, inflammation and Regeneration, 31 (2): 219-227 (2011)

7. Huang YH, et al.: Dental Stem Cells and Tooth Banking for Regenerative Medicine, Journal of Experimental & Clinical Medicine, 2 (3): 111-117 (2010)

8. Lee SY, et al.: Effects of Cryopreservation of Intact Teeth on the Isolated Dental Pulp Stem Cells, Journal of Endodontics, 36 (8): 1336-1340 (2010)

9. Mieno S., et al., Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. J. Card Surg., 25 (5): 618-25 (2010)

10. Saeki K. et al.: High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells, Cloning and Stem Cells, 4: 509-22 (2009).

11. Shimizu Y. et al.: Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages: Cancer Sci., 100 (3): 481-9 (2009)

12. Park HS, et al.: Bone marrow T Cells are superior to splenic T cells to induce chimeric conversion after non-myeloablative bone marrow transplantation, Korean J. Intern Med., 24 (3): 252-262 (2009)

13. Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells, Differentiation, 76 (9): 981-93 (2008)

14. Mieno S., et al.: Characteristics and Function of Cryopreserved Bone Marrow-Derived Endothelial Progenitor Cells. Annals of Thoracic Surgery., 85: 1361-6 (2008)

15. Hikichi T., et al.: Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25: 46-53 (2007)

16. Zaidi S. et al.: Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential. PNAS., 104(50): 19861-19866 (2007)

17. Liu DG et al, Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation, Xenotransplantation, (1): 67-73 (2007)

18. Hatoya S., et al.: Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes, Journal of Theriogenology, 66: 1083-1090(2006)

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