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MicroRNA Research Tools

Nucleic Acid Research

Wako offers a range of Ago antibody based products to study Ago protein expression and investigate the miRNA and mRNA that associate with Ago proteins in the RISC complex.

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Worldwide research efforts continue to identify new types of miRNA and elucidate their function, with roles for miRNAs described in a range of biological functions including organism growth, development and metabolism. Of particular interest are circulating miRNA found in blood and other body fluids that may be associated with disease and could potentially serve as clinical markers for cancer and other diseases. Wako offers a range of antibodies and RNA isolation kits to assist researchers investigating miRNA expression, function and mRNA targets.

Argonaute proteins are of key importance in gene-silencing pathways and are subdivided into Argonaute(Ago); PIWI (named after the drosophiliaprotein P-element-induced wimpy testis); and nematode-specific worm Argonaute (Wago) proteins. Ago proteins appear to be expressed everywhere whilst Piwi protein expression is restricted mainly to the germ line. Wako provide monoclonal Anti-Ago antibodies for number of applications including can be used for a number of applications including western blotting and immunoprecipitation.

  • MicroRNA Research Antibodies and Kits

    • PDF size: 5.03MB
    • Updated on: 04/07/2018

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MicroRNAs are produced from long primary miRNA transcripts, termed pri-miRNAs, which pass through sequential endonucleolytic maturation steps to give precursor miRNAs (pre-miRNAs), typically 70 nucleotide in length, which are processed by the endonuclease Dicer to give the smaller miRNA fragments. The mature miRNAs are localised in the cytoplasm where they bind and assemble into the RNA-induced silencing complex (RISC) to target messenger RNA (mRNA). At the heart of every RISC is a member of the Argonauteprotein family, which serves to bind the miRNAsand position them in a way that facilitates target recognition. The miRNA bind complementary sequences in the target and the RISC may then directly cleave the target RNA or recruit other gene-silencing proteins to inhibit translation (Fig 1).

 

Figure 1.

Molecular_Mechanism_RNA_Silencing

 

Figure 2. Human (A) and mouse (B) recombinant Ago proteins containing a DYKDDDDK tag were detected by western blot using the Wako monoclonal antibody clones indicated as primary and anti-mouse IgG, peroxidase conjugates, as secondary.

Ago proteins containing a DYKDDDDK tag

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