The Phos-tag™ developed by Hiroshima University is a functional molecule that specifically binds phosphorylated ions, forming stable complexes under physiological conditions. Phos-tag acts as a novel labelling system for analysis of phosphorylation.Fujifilm Wako offers series of reagents utilizing Phos-tag™ which can be used in the separation, detection, mass spectrometry analysis, and purification of phosphorylated proteins.
The acrylamide-pendant Phos-tag™ ligand (Phos-tag™ AAL-107) provides a phosphate affinity SDS-PAGE for mobility shift detection of phosphorylated proteins. Phos-tag acrylamide and divalent metal (MnCl2 or ZnCl2) is incorporated into the SDS-PAGE resolv
Phos-tag™ Agarose is used to enrich, separate, and purify phosphorylated proteins using column chromatography. One of their main properties being free of reductants or surfactants, it can refine phosphorylated proteins in their native shape.
The biotin-bound Phos-tag™ is used for the detection of phosphoproteins on PVDF membrane as an alternative to using anti-phosphorylated antibodies.
The Phos-tag™ Mass Analytical Kit can be used to provide a simple, rapid, and sensitive matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) analysis of phosphorylated compounds.
Phos-tag™ fluorescent gel stain reagents for use with SDS-PAGE gels. Fluorescein molecules combined with the "Phos-tag™" that captures phosphate groups(-PO32-) can be used to specifically stain phosphorylated proteins with the gel.