Phos-tag™
The Phos-tag™ developed by Hiroshima University is a functional molecule that specifically binds phosphorylated ions, forming stable complexes under physiological conditions. Phos-tag acts as a novel labelling system for analysis of phosphorylation.
Fujifilm Wako offers series of reagents utilizing Phos-tag™ which can be used in the separation, detection, mass spectrometry analysis, and purification of phosphorylated proteins.
The acrylamide-pendant Phos-tag™ ligand (Phos-tag™ AAL-107) provides a phosphate affinity SDS-PAGE for mobility shift detection of phosphorylated proteins. Phos-tag acrylamide and divalent metal (MnCl2 or ZnCl2) is incorporated into the SDS-PAGE resolv
The biotin-bound Phos-tag™ is used for the detection of phosphoproteins on PVDF membrane as an alternative to using anti-phosphorylated antibodies.
The Phos-tag™ Mass Analytical Kit can be used to provide a simple, rapid, and sensitive matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) analysis of phosphorylated compounds.
Phos-tag™ fluorescent gel stain reagents for use with SDS-PAGE gels. Fluorescein molecules combined with the "Phos-tag™" that captures phosphate groups(-PO32-) can be used to specifically stain phosphorylated proteins with the gel.