Acrylamide

The acrylamide-pendant Phos-tag™ ligand (Phos-tag™ AAL-107) provides a phosphate affinity SDS-PAGE for mobility shift detection of phosphorylated proteins. Phos-tag acrylamide and divalent metal (MnCl2 or ZnCl2) is incorporated into the SDS-PAGE resolv

During electrophoresis the Phos-tag captures the phosphorylated protein as they migrate through the gel, resulting in detectable differences in the mobilities of phosphorylated proteins.

Following the Phos-tag SDS-PAGE further downstream analysis technique can be applied such as fluorescent gel staining or proteins recovered from the gel for analysis by mass spectroscopy.