Using the BÜHLMANN CAST® range of Allergy assays can significantly decrease the amount of provocation tests that need to be performed.
Alpha Laboratories supplies the BÜHLMANN CAST® (Cellular Allergen Stimulation Test) allergy assays in two different formats.
The Flow CAST® flow cytometric assay is quick (1 hour) and easy and uses whole blood for a flow cytometry based Basophil Activation Test (BAT) upon stimulation with the suspected allergen. The ELISA (CAST® ELISA) is also available.
Over 150 BÜHLMANN allergens that are quality controlled, validated and standardized complement the allergy test range.
The new Flow2 CAST® system represents a significant step forward in in
vitro allergy testing, allowing the simple detection of Basophil Activation
on whole blood samples. The Flow2 CAST system, which replaces the
Flow CAST assay, uses a dual labelling system to identify basophils in
whole blood samples, negating the requirement for lengthy and labour
intensive leukocyte isolation steps. Using a flow cytometer, basophils are
identified in the sample by using a specific marker (CCR3) and then the
level of CD63 is analysed on this sub-population to give a read out of
the response to a purified allergen which is added to the sample.
In the CAST-2000® ELISA, leukocytes isolated from patient blood
are simultaneously primed with Interleukin 3 (IL-3) and stimulated
with allergens. The ELISA measures de novo synthesis of the allergic
sulfidoleukotriene mediators (LTC4, LTD4 and LTE4) as a quantitative
read-out of the patient’s response to the allergen.
The Flow2 CAST system, together with the CAST 2000 ELISA, is particularly suited for the
following types of allergies:
- Drug hyper-sensitivites (e.g. anaesthetics, antibiotics, NSAIDs)
- Food allergies
- Bee and wasp stings
The BÜHLMANN CAST® assays detect immediate type allergic reactions and hypersensitivities to suspected allergens.
In some cases, specific IgE fails to demonstrate absolute diagnostic reliability. This happens mainly in more complex reactions such as drug, venom or food allergies. The CAST® (Cellular Allergen Stimulation Test) allergy assays will identify both IgE and non-IgE allergies.
Road map for the clinical application of the basophil activation test in food allergy
Santos, A.F. and Shreffler, W.G. Clin. & Exp. Allergy Volume 47, Issue 9, September 2017, Pages 1115–1124
Basophil activation test discriminates between allergy and tolerance in peanut-sensitized children
Santos at al. Journal of Allergy and Clinical Immunology;Volume 134, Issue 3, September 2014, Pages 645–652
Diagnosis of Immediate-Type ß-Lactam Allergy In Vitro by Flow-Cytometric Basophil Activation Test and Sulfi doleukotriene Production: A Multicenter Study
De Weck at al. J Investig Allergol Clin Immunol;Volume 19, Issue 2, 2009, Pages 91-109