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Transfection

ScreenFect™A is a cationic lipid transfection reagent combining high transfection efficiency with low cell toxicity.

Offering a simple one-step protocol which combines both transfection and plating of cells, ScreenFectA can be used to transfect cells with DNA or siRNA.

Transfection can typically be detected in 24-48h and the low cell toxicity of ScreenFectA eliminates the need to change media post-transfection.

Efficient transfection for DNA and siRNA

ScreenFect A is a new lipid based transfection reagent for use with plasmid DNA or siRNA.  Using Click Chemistry a library of newly developed cationic liposomes were synthesised and then screened in vitro to determine transfection efficiency.  When transfecting a GFP expressing plasmid into HEK 293T cells ScreenFect A demonstrated the best transfection efficiency and exhibits minimal toxicity.

Simple One-Step Protocol

ScreenFect A offers a simple one-step protocol which combines both transfection and plating of cells.   Cells from the logarithmic growth phase are detached from their routine culture vessel and suspended in medium.  Diluted ScreenFect A and DNA/siRNA complex is then added to the cell suspension and mixed gently by pipetting before transfer to the plate. 

Transfection results can typically be detected in 24-48h.  The low cell toxicity of ScreenFect A eliminates the need to change media post-transfection and since ScreenFect A can be added directly to media containing serum and antibiotics transfection protocols can be fuss-free.

Cryoprotectant solutions

Transfection Efficiency that Stands Out from the Crowd

ScreenFect A demonstrated transfection efficiency equal to or better than other commercially available transfection reagents.  Seen below are HEK 293 cells transfected with 75ng/well of GFP-expressing plasmid in a 96 well culture plate. 

GFP Vs Hoechst

Efficient siRNA Delivery

ScreenFect A mediated siRNA delivery has also been demonstrated.  The efficiency of GAPDH siRNA transfection using ScreenFect A and a competitor product was compared using HeLa, MCF7 and A431 cell lines.   Cells were transfected with 3nM/well GAPDH siRNA in 96 wells efficiency assessed by mRNA quantification. 

siRNA-Concentration

Suppression of low-density lipoprotein receptor (LDL-R) - related protein 6 (LRP-6) using siRNA was investigated in HEK 293 cells following transfection with different reagents.   Cells were transfected in 96 well plates with 2 nM/well LRP6 siRNA targeting LRP-6.  ScreenFect A mediated delivery of the siRNA resulted in greated protein knock-down than competitor products.

ScreenFectA Dilution Buffer

Transfection Success with Wide Range of Cell Lines

The ScreenFect A formula has been successfully used to transfect DNA or siRNA into a broad range of cells with high efficiency.  Not just commonly used cell lines such as HeLa, HepG2 and Cos-7 but also blood cells (macrophages, THP-1 and RAW264.7), microglia and primary cell cultures.  Success has even been reported with stem cell cultures (mouse embryonic stem cells (MES) and embryonic fibroblasts (MEF)).  Seen below ScreenFect A mediated transfection of GFP in MES, demonstration 60% efficiency.    

GFP brightfield

Fuss-Free Transfection

  • High transfection efficiency
  • Low toxicity – no need to change medium after transfection
  • Serum compatible
  • for use with DNA and siRNA

 

  • ScreenFect™A

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    Perfecting your transfection of DNA and siRNA

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Li et al, 2012: A biomimetic lipid library for gene delivery through thiol-yne click chemistry 

Fischer et al, 2013:  Breaking limitation of complex culture media: functional non-viral miRNA delivery into pharmaceutical production cell lines

Liu et al, 2013: Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi-specific monoclonal antibody MsMab-1 

Enlund et al, 2014: Establishment of lipofection for studying miRNA function in human adipocytes

Diefenbacher et al, 2014: The LIM domain protein nTRIP6 recruits the mediator complex to AP-1-regulated promoters 

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