Fluorescent Gel Stain Reagent
Phos-tag™ fluorescent gel stain reagents for use with SDS-PAGE gels. Fluorescein molecules combined with the "Phos-tag™" that captures phosphate groups(-PO32-) can be used to specifically stain phosphorylated proteins with the gel.
Because these stains can be used at physiological pH, it allows detection of proteins that are phosphorylated at histidine (pHis) and aspartic acid (pAsp) residues, which are susceptible to hydrolysis under acidic conditions.
Wako offers four different fluorescent reagents with different fluorescence wavelengths.