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Phos-tag™ Cyan fluorescent gel stain for detection of phosphorylated protein and peptide residues 0.2mg NARD Institute FUJIFILM Wako Chemicals

Product code: 389-15211

Pack Size: 0.2mg
CE Verified: Research Use Only
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Product Information

Phos-tag™ Cyan 0.2 mg manufactured by Nard Institute Ltd and distributed in association with FUJIFILM Wako Chemicals

Phos-tag™ is a novel labelling system for analysis of phosphorylation developed by the Department of Functional Molecular Science at Hiroshima University. The functioning Phos-tag molecule contains two divalent metal ions and recognises the phosphate monoester group.
Phos-tag fluorescent gel stain reagents for SDS-PAGE gels specifically stain phosphorylated proteins. They can be used at physiological pH 7-8 where no dephosphorylation occurs enabling detection of proteins phosphorylated at histidine (pHis) and aspartic acid (pAsp) which are susceptible to hydrolysis under acidic conditions.

•Simple 3 step protocol : Fixing - Staining – Washing
•Stain gels in 2 hours
•Detection limit of ovalalbumin >1ng/lane
•Staining at physiological pH
•High selectively to pSer, pTyr, pHis, and pAsp residues
•No need for radioactive labelling

Each vial of Phos-tag Cyan can be used to stain up to 20 mini gels.

Ex/Em Wavelength = 643/661 nm

Once reconstituted, Phos-tag Cyan Stain Solution should be stored at 4°C in the dark using a polypropylene or polyethylene vessel (to avoid adsorption that will diminish fluorescence intensity) and used within 6 months. Preparation of Stain Solution requires Mixed reagents for Phos-tag Common Solution 5X (383-15231), methanol and pure water.


  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Application Detection of phosphorylated protein and peptide residues is SDS-PAGE gels
  • Contents 0.2mg Phos-tag Cyan to be used with Mixed reagents for Phos-tag common solution (x5) to create fluorescent gel stain

Storage Details

  • Pack Description 0.2mg
  • Shipping Condition Blue Ice
  • Storage Condition Fridge
  • Pack Length (m) 0.1
  • Pack Width (m) 0.05
  • Pack Height (m) 0.15
  • Pack Weight (kg) 0.00

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