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Negative gel stain post-electrophoresis staining Wako
Pack size: 20 Tests
Product code: 293-57701

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Negative gel stain post-electrophoresis staining Wako

Negative Gels Stain MS Kit produced by FUJIFILM Wako Chemical Corporation



Protein bands separated by SDS-PAGE can be visualised as transparent bands against a background of milky white stained gel using a zinc imidazole reagent. The Wako Negative Gels Stain MS Kit utilises a new imidazole derivative. Free zinc ions will react with protein and SDS-protein complexes. These areas of the gel remain clear but insoluble zinc-imidazole complex is deposited elsewhere giving a negative stain to the gel. This staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues – important for subsequent studies such as protein sequencing and mass spectrometry analysis.



Negative Gels Stain MS Kit 20 Tests



Highly sensitive 3ng



Quick detection 10 min



Ideal for subsequent down-stream protein analysis



Suitable for repeated staining and de-staining



After de-staining the gel may be subject to Western blotting



Each kit contains:

500ml each of Staining Solution A, Staining Solution B and De-staining Solution.



Procedure:

Immerse gel in Solution A with gentle shaking for 5-10min

Drain off the stain solution and wash in deionised water 3 x 1min

Drain and add Solution B, shake gentle for 10-50s whilst checking the staining intensity against a black background.

Stop staining by draining off the solution and washing in distilled water 3 x 1min

In case of excess staining the gel can be de-stained with 2% EDTA3Na.

For subsequent processes such as Western Blotting the gel is de-stained by immersion in the De-staining Solution provided with gentle stirring, complete removal of the stain typically takes 15min



Store kit at room temperature
Brand
FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
Sterile
No
CE Certified
Research Use Only
Warranty Period
Standard Terms Apply
Application
SDS-PAGE gel stain
Form
20 Tests
Marketed Grade
Electrophoresis Grade
UNSPSC Description
Dyes
UNSPSC Code
12171500
NHS eClass Description
Chemicals & Reagents
NHS eClass Code
HXB
Pack Description
20 Tests
Shipping Condition
Ambient
Storage Condition
Special
Pack Length (m)
0.1
Pack Width (m)
0.16
Pack Height (m)
0.5
Pack Weight (Kg)
0.5000

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  • Protein Markers - Size markers for protein electrophoresis

    • PDF size: 1.17MB
    • Updated on: 04/07/2018

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