Charomid 9-42 DNA vector cosmid 9 cloning sites length 42.2kb insert 0-10kb gene genomic library construction high efficiency Nippon Gene Wako

Charomid 9-42 DNA produced by Nippon Gene and distributed via Wako Pure Chemicals.

Charomids are cosmid vectors bearing multiple copies of a 2kb spacer fragment. Like λ phage, charomids can be packaged in vitro for efficient introduction into bacteria offering high cloning efficiency comparable to that of lambda vectors. The Charomid vectors have 9 cloning sites (Aat II, EcoR I, Sac I, Sma I, Xba I, Apa I, Hind III), for charomid vectors of 28kb and 36kb there is no need to prepare vector arms, as may be necessary for some lambda vectors. Partial digestion should not increase the number of clones without inserts. Include AMPr gene for ampicillin tolerance. After isolation of a clone, the vector size can be reduced to 2.9kb by deletion of the spacer sequences.

Charomid Vector: Charomid 9-42 DNA 10µg
Length 42.2kb
Spacers: 18
Capacity for insert: 0-10kb
Store: -20C

Imperial Cancer Research Fund have sole ownership of Charomid vectors.