Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.
Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.
The BÜHLMANN fCAL® ELISA is ideal for use in high through put laboratory environments for screening and monitoring IBD patients. The EK-CAL2-WEX does not contain any buffer and is designed for use with the CALEX Cap extraction device (B-CALEX-C50 or B-CALEX-C200) which can be used directly on the ELISA processor:
Sample Type: Faeces
Test Time: 75 minutes
Assay Range: 30 - 1800µg/g or 10 - 600µg/g (Different test protocol to achieve the higher or lower range)
Number of wells: 2 x 96 Each plate is individually sealed (up to 80 samples in duplicate)
Assay protocols: DS2 and Triturus
All reagents are ready to use (except the wash buffer)
No Extraction Buffer included
Calibrators and controls are included
Analytical Sensitivity: < 10µg/g Calprotectin in faecal sample
Established cut-off of 50µg/g