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Faecal calprotectin Immuno turbidimetric assay extraction buffer BÜHLMANN fCAL® turbo for differentiating IBS vs IBD and disease monitoring.
Pack size: Each
Product code: B-CAL-EX12

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Faecal calprotectin Immuno turbidimetric assay extraction buffer BÜHLMANN fCAL® turbo for differentiating IBS vs IBD and disease monitoring.

Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.

Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.

The BÜHLMANN fCAL® turbo is ideal for use in high throughput laboratory environments for screening and monitoring IBD patients and can be used on many open access biochemistry analysers. It is a turbidimetric immunoassay based on the standardisation of the BÜHLMANN fCAL® ELISA:

Code: B-KCAL-EX12 (12 x 125ml)

Sample Type: Faeces

Sample preparation: CALEX or weighing with 1:500 dilution

Sample volume: 10µl of 1:500 extract

Time to first result: 10 minutes

Assay Range: 20 - 8000µg/g

Assay protocols available: Siemens Advia 1650, Advia 1800, Advia 2400, Advia XPT, Roche Cobas 6000 (c501), Abbott Architect, Mindray BS200e, Mindray BS380

All reagents are ready to use and have an on board stability of 60 days

Calibration is stable for 60 days

Analytical Sensitivity: 6µg/g Calprotectin in faecal sample

Limit of Quantification: 11µg/g

Established cut-off of 50µg/g = Negative, >200µg/g = Positive
Brand
BÜHLMANN
Sterile
No
CE Certified
CE IVD
Warranty Period
Standard Terms Apply
Application
extraction of calprotectin from stool samples
Shelf Life
365
Preparation Form
Liquid
UNSPSC Description
Chemistry reagents or solutions
UNSPSC Code
41116105
NHS eClass Description
Buffers
NHS eClass Code
HDB
Pack Description
Each
Shipping Condition
Ambient
Storage Condition
Fridge
Pack Length (m)
0.22
Pack Width (m)
0.16
Pack Height (m)
0.11
Pack Weight (Kg)
0.1000

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