Cell Freezing Media
BAMBANKER™ serum-free cell freezing media is specially formulated to minimise cell damage during the freezing process and improve survival rates. With the BAMBANKER™ Direct format this just got quicker and easier.
Applications
Cryogenic Storage
Storing banks of valuable cell lines for future use and reference is essential for many researchers and the technique of cryopreservation is a mainstay for numerous labs. By cooling cells to sub-zero temperatures biological activity is effectively stopped. But ultra low temperature storage is not without risk and cells can incur freezing injuries that compromise viability after thawing.
Freezing Cells
Cells can be directly damaged by the formation of ice crystals. When cooled slowly water migrates out of the cells forming ice in the extracellular spaces, too much ice can cause mechanical damage to the cell membrane and crush the cell. Similarly, intracellular ice formation can disrupt organelles and cause lethal damage. As water freezes, solutes such as salt are excluded and become concentrated in the remaining liquid which can prove toxic. The migration of water can also result in cellular dehydration.
Cryoprotectant Solutions
Adaptive mechanisms to protect organisms exposed to very low temperatures can be observed in the natural world. Microscopic multicellular organisms called ‘Water-bears’ (Tardigrada) survive freezing by replacing most of their internal water with the sugar trehalose, preventing it from crystallisation that would damage cell membranes. North American wood frogs have also evolved freezing survival strategies; they accumulate urea in tissues in preparation for overwintering and increase release of glucose from the liver to prevent internal ice formation. Using solute solutions as cryoprotectants can significantly reduce the risks of freezing injury.
Cell Freezing Media
The unique, patented formulation of BAMBANKER™ serum-free cell freezing medium has been optimised to give excellent cell recovery. Developed by Lymphotec Inc to preserve even delicate cells during medium and long term ultra low temperature storage, BAMBANKER has been tested with established cell lines, primaries and stem cells. Both human and animal cells have been successfully preserved, demonstrating excellent survival rates.
Benefits
Cryogenic preservation of cell cultures is a popular way to maintain back up cultures and reserves of cell lines.
Bambanker is ready-to use, no dilution or addition of DMSO/glycerol are required. Simply harvest cells during the logarithmic growth phase, aspirate media and resuspend in BAMBANKER before freezing.
There is no need for programmable freezers or step-wise temperature reduction just freeze cells directly at -80C. Frozen cells can subsequently be transferred to liquid nitrogen storage facilities if desired.
Now there is no need to spin down and isolate cells before freezing. BAMBANKER Direct can be added directly to the media and frozen straight away, removing a step in the freezing process.
A stringent quality assurance programme ensures protection for your precious cell cultures with sterility, endotoxin, mycoplasma, fungi and bacteria testing on every batch.
Available in two pack sizes, a single 120ml bottle for high volume users storing large batches of cells or 20ml bottles.
Ideal for dedicated use with specific cell lines or culture areas to minimise cross contamination of cultures and culture facilities.
Ready to use
Freeze and store at -80C
Stringent QA including mycoplasma testing
Serum-free
Choice of pack sizes
HeLaS3 Cells Cryopreservation Data
Before freezing
3 days after thawing
Publications
More References Featuring BAMBANKER
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15. Hikichi T., et al.: Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells, 25: 46-53 (2007)
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