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Lipid transfection reagent DNA siRNA ScreenFect™ A 1ml Incella Wako
Product code: 299-73203
Pack Size: 1ml
CE Verified: Research Use Only
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
ScreenFect™ A produced by FUJIFILM Wako Chemical Corporation
ScreenFect™ A is a transfection agent for use with DNA or siRNA. It combines high transfection efficiency with low cell toxicity - no need to change media after transfection. The transfection properties of ScreenFect A were discovered by screening a biomimetic lipid library of thioethether lipids synthesizes by click chemistry. ScreenFect A is designed for use with cells in the exponential growth phase and can be used to transfect DNA or siRNA in a simple one-step protocol. Cells to be transfected are detached from their routine culture vessel and mixed with the ScreenFect A and DNA/siRNA complexes before transfer to a new culture dish or plate. Because ScreenFect A has low cell toxicity there is no need to change the culture media after transfection. ScreenFect A will even work in medium supplemented with serum so need to shock cells prior to transfection by switching to serum-free culture. ScreenFect A has been successfully used to transfects common cells lines such as HeLa, HEK293 and Cos-7, as well as more specialised cultures such as mouse embryonic stem cells, blood macrophages, microglia and primary cultures.
Some cells may require a two-step protocol to increase transfection efficiency.
ScreenFect A : 1ml transfection reagent, 50ml dilution Buffer
Store at 4C
ScreenFect™ A is a transfection agent for use with DNA or siRNA. It combines high transfection efficiency with low cell toxicity - no need to change media after transfection. The transfection properties of ScreenFect A were discovered by screening a biomimetic lipid library of thioethether lipids synthesizes by click chemistry. ScreenFect A is designed for use with cells in the exponential growth phase and can be used to transfect DNA or siRNA in a simple one-step protocol. Cells to be transfected are detached from their routine culture vessel and mixed with the ScreenFect A and DNA/siRNA complexes before transfer to a new culture dish or plate. Because ScreenFect A has low cell toxicity there is no need to change the culture media after transfection. ScreenFect A will even work in medium supplemented with serum so need to shock cells prior to transfection by switching to serum-free culture. ScreenFect A has been successfully used to transfects common cells lines such as HeLa, HEK293 and Cos-7, as well as more specialised cultures such as mouse embryonic stem cells, blood macrophages, microglia and primary cultures.
Some cells may require a two-step protocol to increase transfection efficiency.
ScreenFect A : 1ml transfection reagent, 50ml dilution Buffer
Store at 4C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Contents 1ml transfection reagent,50ml dilution buffer
Storage Details
- Pack Description 1ml
- Shipping Condition Blue Ice
- Storage Condition Fridge
- Pack Length (m) 0.1
- Pack Width (m) 0.6
- Pack Height (m) 0.6
- Pack Weight (kg) 0.50
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