Acrylamide

The acrylamide-pendant Phos-tag™ ligand (Phos-tag™ AAL-107) provides a phosphate affinity SDS-PAGE for mobility shift detection of phosphorylated proteins. Phos-tag acrylamide and divalent metal (MnCl2 or ZnCl2) is incorporated into the SDS-PAGE resolv

During electrophoresis the Phos-tag captures the phosphorylated protein as they migrate through the gel, resulting in detectable differences in the mobilities of phosphorylated proteins.

Following the Phos-tag SDS-PAGE further downstream analysis technique can be applied such as fluorescent gel staining or proteins recovered from the gel for analysis by mass spectroscopy.

Phos-tag™ Acrylamide AAL-107 aqueous solution 5mM phos-tag phosphate-affinity SDS-PAGE electrophoresis Wako Nard Institute 0.3ml
Phos-tag™ Acrylamide AAL-107 phosphate tag phosphate-affinity SDS-PAGE electrophoresis Wako Nard Institute 10mg
Phos-tag™ Acrylamide AAL-107 phosphate tag phosphate-affinity SDS-PAGE electrophoresis Wako Nard Institute 2mg
Phos-tag™ SuperSep™ 12.5% acrylamide 17 well pre-cast polyacrylamide gel 50µmol/L phos-tag XCell PAGE electrophoresis NARD Wako
Phos-tag™ SuperSep™ 6% acrylamide 17 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako
Phos-tag™ SuperSep™ 7.5% acrylamide 17 well pre-cast polyacrylamide gel 50µmol/L phos-tag BioRad PAGE electrophoresis NARD Wako
Phos-tag™ SuperSep™ 15.0% acrylamide 17 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako
Phos-tag™ SuperSep™ 12.5% acrylamide 17 well pre-cast polyacrylamide gel 50µmol/L phos-tag BioRad PAGE electrophoresis NARD Wako
Phos-tag™ SuperSep™ 7.5% acrylamide 13 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako
Phos-tag™ SuperSep™ 12.5% acrylamide 13 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako
Phos-tag™ SuperSep™ 10% acrylamide 13 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako
Phos-tag™ SuperSep™ 15.0% acrylamide 13 well pre-cast polyacrylamide gel 50µmol/L phos-tag PAGE electrophoresis NARD Institute Wako