in vitro Allergy Provocation Test

Mediators of allergy, such as leukotrienes or histamine are produced and/or secreted upon contact of allergen with IgE molecules on specialized cells such as basophils and mast cells.

In contrast to histamine, leukotrienes are also synthesized and released in the case of the pseudo-allergies, reactions resembling allergy, but without involvement of any IgE molecules.


The CAST® assays can be used for the confirmation of any IgE mediated allergy with controversial results from classical testing (anamnese, skin testing, spec. IgE).

The CAST® ELISA is an in vitro allergy provocation test. The collected patient's leukocyte blood fraction is exposed in vitro to the suspected specific allergen. After incubation the cellular supernatant will be tested for the de novo synthesis of sulfidoleukotrienes by a highly specific ELISA.

Leukocytes isolated from patient blood are simultaneously primed with Interleukin 3 (IL-3) and stimulated with allergens. The ELISA measures de novo synthesis of the allergic sulfidoleukotriene mediators (LTC4, LTD4 and LTE4) as a quantitative read-out of the patient’s response to the allergen.

Product Information

Method ELISA
Method ELISA Time to Result 5.5 h (approx.)
Method ELISA Sample Type 200 µl EDTA blood
Method ELISA Standard Range 50-3200 pg/ml
Method ELISA Sensitivity 19 pg/ml

The CAST ELISA is CE Marked