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Negative gel stain post-electrophoresis staining Wako
Product code: 293-57701
Pack Size: 20 Tests
CE Verified: Research Use Only
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Product Information
Negative Gels Stain MS Kit produced by FUJIFILM Wako Chemical Corporation
Protein bands separated by SDS-PAGE can be visualised as transparent bands against a background of milky white stained gel using a zinc imidazole reagent. The Wako Negative Gels Stain MS Kit utilises a new imidazole derivative. Free zinc ions will react with protein and SDS-protein complexes. These areas of the gel remain clear but insoluble zinc-imidazole complex is deposited elsewhere giving a negative stain to the gel. This staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues – important for subsequent studies such as protein sequencing and mass spectrometry analysis.
Negative Gels Stain MS Kit 20 Tests
Highly sensitive 3ng
Quick detection 10 min
Ideal for subsequent down-stream protein analysis
Suitable for repeated staining and de-staining
After de-staining the gel may be subject to Western blotting
Each kit contains:
500ml each of Staining Solution A, Staining Solution B and De-staining Solution.
Procedure:
Immerse gel in Solution A with gentle shaking for 5-10min
Drain off the stain solution and wash in deionised water 3 x 1min
Drain and add Solution B, shake gentle for 10-50s whilst checking the staining intensity against a black background.
Stop staining by draining off the solution and washing in distilled water 3 x 1min
In case of excess staining the gel can be de-stained with 2% EDTA3Na.
For subsequent processes such as Western Blotting the gel is de-stained by immersion in the De-staining Solution provided with gentle stirring, complete removal of the stain typically takes 15min
Store kit at room temperature
Protein bands separated by SDS-PAGE can be visualised as transparent bands against a background of milky white stained gel using a zinc imidazole reagent. The Wako Negative Gels Stain MS Kit utilises a new imidazole derivative. Free zinc ions will react with protein and SDS-protein complexes. These areas of the gel remain clear but insoluble zinc-imidazole complex is deposited elsewhere giving a negative stain to the gel. This staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues – important for subsequent studies such as protein sequencing and mass spectrometry analysis.
Negative Gels Stain MS Kit 20 Tests
Highly sensitive 3ng
Quick detection 10 min
Ideal for subsequent down-stream protein analysis
Suitable for repeated staining and de-staining
After de-staining the gel may be subject to Western blotting
Each kit contains:
500ml each of Staining Solution A, Staining Solution B and De-staining Solution.
Procedure:
Immerse gel in Solution A with gentle shaking for 5-10min
Drain off the stain solution and wash in deionised water 3 x 1min
Drain and add Solution B, shake gentle for 10-50s whilst checking the staining intensity against a black background.
Stop staining by draining off the solution and washing in distilled water 3 x 1min
In case of excess staining the gel can be de-stained with 2% EDTA3Na.
For subsequent processes such as Western Blotting the gel is de-stained by immersion in the De-staining Solution provided with gentle stirring, complete removal of the stain typically takes 15min
Store kit at room temperature
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Source Species NULL
- CAS Number NULL
- Application SDS-PAGE gel stain
- Molecular Formula NULL
- Form 20 Tests
- Concentration NULL
- Purity NULL
- Marketed Grade Electrophoresis Grade
- Molecular Weight NULL
- Shelf Life NULL
- Species Reactivity NULL
- Preparation Form NULL
Storage Details
- Pack Description 20 Tests
- Shipping Condition Ambient
- Storage Condition Special
- Pack Length (m) 0.1
- Pack Width (m) 0.16
- Pack Height (m) 0.5
- Pack Weight (kg) 0.00