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Denaturing Gradient Gel Electrophoresis DGGE molecular Marker IV 8 Fragments 150µl 16S rDNA mutation bacteria fungi nematode Nippon Gene Wako
Product code: 318-06933
Pack Size: 30 Tests
CE Verified: Research Use Only
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Product Information
DGGE Marker IV (8 Fragments) molecular marker produced by Nippon Gene and distributed in association with Wako Pure Chemicals
DGGE Markers are DNA standards for use with denaturing gradient gel electrophoresis (DGGE). DGGE is a type of electrophoresis in which a constant heat and increasing concentration of denaturing chemicals are used to force DNA molecules to unwind or ‘melt’. Melting domains are the result of a stretch of base pairs with identical melting temperature, any variations of DNA sequence within these melting domains will result in different melting temperatures enabling the sequences to migrate at different positions within the gel. This enables the technique to distinguish between wild type and mutated sequences.
DGGE Marker IV contains 8 fragments, a GC clamp is added at the end of one side of each fragment to enable separation /inspection by DGGE. This molecular marker may be useful for analysis of mutations in fungal and bacterial 16S rDNA. Developed by the National Institute for Agro-Environmental Sciences (NIAES) for the analysis of flora/fauna of bacteria, fungi and nematodes in soil*.
Polyacrylamide gels containing these standards can be stained with ethidium bromide, SYBR green or SYBR Gold.
Concentration 80ng/5µl
150µl for 30 applications
Store at -20C.
*NIAES conducted a project of “Development of soil biodiversity analysis system with environmental DNA” supported by the Ministry of Agriculture, Forestry and Fisheries, Japan. For further information see the journal of the Japanese Society of Soil Microbiology, “Soil and Microorganism”, Vol 62, No.1 April 2008.
DGGE Markers are DNA standards for use with denaturing gradient gel electrophoresis (DGGE). DGGE is a type of electrophoresis in which a constant heat and increasing concentration of denaturing chemicals are used to force DNA molecules to unwind or ‘melt’. Melting domains are the result of a stretch of base pairs with identical melting temperature, any variations of DNA sequence within these melting domains will result in different melting temperatures enabling the sequences to migrate at different positions within the gel. This enables the technique to distinguish between wild type and mutated sequences.
DGGE Marker IV contains 8 fragments, a GC clamp is added at the end of one side of each fragment to enable separation /inspection by DGGE. This molecular marker may be useful for analysis of mutations in fungal and bacterial 16S rDNA. Developed by the National Institute for Agro-Environmental Sciences (NIAES) for the analysis of flora/fauna of bacteria, fungi and nematodes in soil*.
Polyacrylamide gels containing these standards can be stained with ethidium bromide, SYBR green or SYBR Gold.
Concentration 80ng/5µl
150µl for 30 applications
Store at -20C.
*NIAES conducted a project of “Development of soil biodiversity analysis system with environmental DNA” supported by the Ministry of Agriculture, Forestry and Fisheries, Japan. For further information see the journal of the Japanese Society of Soil Microbiology, “Soil and Microorganism”, Vol 62, No.1 April 2008.
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Increments 8 fragments
- Quantitative 16S rDNA mutation analysis
- Type DGGE
- Size 150µl
- Concentration 80ng/5µl
Storage Details
- Pack Description 30 Tests
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.05
- Pack Width (m) 0.05
- Pack Height (m) 0.07
- Pack Weight (kg) 0.00
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