Denaturing Gradient Gel Electrophoresis DGGE molecular Marker III 10 Fragments 30µl16S rDNA mutation bacteria fungi nematode Nippon Gene Wako

DGGE Marker III (10 Fragments) molecular marker produced by Nippon Gene and distributed in association with Wako Pure Chemicals

DGGE Markers are DNA standards for use with denaturing gradient gel electrophoresis (DGGE). DGGE is a type of electrophoresis in which a constant heat and increasing concentration of denaturing chemicals are used to force DNA molecules to unwind or ‘melt’. Melting domains are the result of a stretch of base pairs with identical melting temperature, any variations of DNA sequence within these melting domains will result in different melting temperatures enabling the sequences to migrate at different positions within the gel. This enables the technique to distinguish between wild type and mutated sequences.

DGGE Marker III contains 10 fragments, a GC clamp is added at the end of one side of each fragment to enable separation /inspection by DGGE. This molecular marker may be useful for analysis of mutations in bacterial 16S rDNA. Developed by the National Institute for Agro-Environmental Sciences (NIAES) for the analysis of flora/fauna of bacteria, fungi and nematodes in soil*.
Polyacrylamide gels containing these standards can be stained with ethidium bromide, SYBR green or SYBR Gold.

Concentration 100ng/5µl

30µl for 6 applications

Store at -20C.

*NIAES conducted a project of “Development of soil biodiversity analysis system with environmental DNA” supported by the Ministry of Agriculture, Forestry and Fisheries, Japan. For further information see the journal of the Japanese Society of Soil Microbiology, “Soil and Microorganism”, Vol 62, No.1 April 2008.