Anti-Isocitrate dehydrogenase 1 human hamster IDH1 mouse monoclonal primary antibody clone RMab-3 ELISA Western Blotting WB Immunochemistry IC Wako

Anti-Isocitrate Dehydrogenase 1 (IDH1) produced by Wako Pure Chemicals.
Isocitrate dehydrogenase (IDH) is an oxidoreductase that catalyses the oxidative decarboxylation of isocitrate producing α-ketoglutarate and carbon dioxide. There are three isoforms of IDH, IDH3 is NAD+ dependent and catalyses the third step of the tricarboxylic acid (TCA) cycle within the mitochondria matrix. The IDH1 and IDH2 isoforms use NADP+ as a cofactor and are primarily located in the cytoplasm and mitochondria respectively. In addition to their catabolic potential in the TCA cycle the IDH1 and IDH2 isoforms also serve to shuttle electrons between the mitochondria and cytosol and play a role in cellular defence against oxidative damage. Mutations in the IDH1 and IDH2 isoforms have been reported in some cancers.

Gliomas are tumours that start in the brain or spine with varying malignancy. Astrocytomas are one of the most common forms of primary brain tumour that develop in astrocytes, an abundant subset of glial cells in the nervous system that serve a number of structural and biochemical support roles. There is a WHO classification for grading astrocytomas with regard to malignancy from Pilocytic astrocytoma (Grade I) and Diffuse astrocytoma (Grade II) to Anaplastic astrocytoma (Grade III) and Glioblastoma (Grade IV).

Frequent mutations of Isocitrate dehydrogenase (IDH) have been reported in grad II/III glioma or in stage IV glioblastoma that has developed from low-grade gliomas, most commonly the mutations are seen in IDH1 (arginine residue 132) and IDH2 (arginine residue 172). Other IDH1 mutations include R132H, R132S, R132G, R132C and R132L.

This monoclonal antibody detects wild type and mutated IDH1 suitable for use in ELISA, Western Blot and immunochemistry applications.

Cross reacts with human, mouse and hamster IDH1.

Clone: RMab-3

Host: Mouse

Isotype: IgG₁

Source: Purified from hybridoma supernatant using protein G agarose

Concentration: indicated on vial label

Formulation: PBS (pH 7.2) containing 0.05% sodium azide

Suggested dilutions:
ELISA: 1-5µg/ml
Western Blot: 5µg/ml
Immunohistochemistry: 5-10µg/ml
Store -20°C.