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Anti Asialo GM1 rabbit antiserum lyophilised serum immunodepletion mouse and rat natural killer NK activity Wako

Product code: 986-10001

Pack Size: 1ml
CE Verified: Research Use Only
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.

Product Information

Anti Asialo GM1 rabbit antiserum for immunodepletion of mouse and rat natural killer NK activity manufacture by Wako Pure Chemicals.

Anti Asialo GM1 antiserum has been shown to eliminate natural killer (NK) activity in cells of various strains of mice and rats. The Anti-Asialo GM1 antiserum was raised in rabbit following repeated immunization with purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund's adjuvant.

The gammaglobulin fraction of serum was obtained by 50% ammonium sulphate precipitation methods followed by dialysis with phosphate buffered saline (pH 7.2). Anti Asialo GM1 reacts with mouse and rat NK cells; mouse monocytes (liver cells which contain no NK cells; bone marrow; fetal liver cells; spleen cells of nude mice-macrophages); fetal thymocytes (12 days old; ratio of existence decreased gradually until there were none in new-born mice).

The antibody is supplied lyophilised and should be reconstituted with 1ml distilled water, other solvents such as PBS or MEM may increase the salt concentration.

Antibody titre by immunoflocculation test is approximately 1:1000

Each package comes with lot specific in vivo and in vitro titration data.

Store at 2-10C.

For Research Use Only.

Specification

  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Host Rabbit
  • Clonality polyclonal
  • Application Immunodepletion of rat or mouse NK cells, in vivo or in vitro
  • Form lyophilised serum to make 1ml
  • Specificity Mouse and rat NK cells; mouse monocytes (liver cells which contain no NK cells; bone marrow; fetal liver cells; spleen cells of nude mice-macrophages); fetal thymocytes
  • Preparation Form Gammaglobulin fraction was obtained by 50% ammonium sulphate precipitation followed by dialysing with PBS (pH 7.2)
  • Concentration lot dependent
  • Clone Number NULL
  • Isotype NULL
  • Source Species Rabbit
  • Shelf Life NULL
  • Cross Reactivity NULL
  • Immunogen purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund's adjuvant

Storage Details

  • Pack Description 1ml
  • Shipping Condition Blue Ice
  • Storage Condition Fridge
  • Pack Length (m) 0.0015
  • Pack Width (m) 0.0015
  • Pack Height (m) 0.0022
  • Pack Weight (kg) 0.00

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