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Anti-Green Fluorescent Protein GFP mouse monoclonal primary antibody immunoprecipitation IP immunocytochemistry ICC clone mFX73 100µl Wako
Product code: 012-20461
Pack Size: 100µl
CE Verified: Research Use Only
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Product Information
Anti-Green Fluorescent Protein (GFP) produced by Wako Pure Chemicals.
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice.
Wako offers a mouse monoclonal antibody for immunoprecipitation or immunohistochemistry of native GFP and GFP variants. This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP's partial peptide coupled with BSA after being immunized with GFP.
The hybridoma supernatant cultured by protein free culture was subjected to ultrafiltration with an ultra-filter 30,000-molecular-weight cut-off and prepared in PBS solution containing 10 mmol/L HEPES and 10 mmol/L sodium azide
Anti-Green Fluorescent Protein (GFP) Clone mFX73 100µl
Mouse IgG2a.
Suitable for Immunoprecipitation (IP) and Immunostaining (Immunohistochemistry, IHC) applications.
Suggested dilutions for: Immunoprecipitation (IP): 1-5µg/5µl sepharose
Immunostaining: 0.5-1µg/ml
Store at -20°C.
For research use only.
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been used with increasing success as a marker protein for gene expression, intracellular processes and localization ranging from bacteria to transgenic mice.
Wako offers a mouse monoclonal antibody for immunoprecipitation or immunohistochemistry of native GFP and GFP variants. This monoclonal antibody is produced by a hybridoma, which is established by fusing mouse myeloma cells, NSO and spleen cells of BALB/c mice immunized with the GFP's partial peptide coupled with BSA after being immunized with GFP.
The hybridoma supernatant cultured by protein free culture was subjected to ultrafiltration with an ultra-filter 30,000-molecular-weight cut-off and prepared in PBS solution containing 10 mmol/L HEPES and 10 mmol/L sodium azide
Anti-Green Fluorescent Protein (GFP) Clone mFX73 100µl
Mouse IgG2a.
Suitable for Immunoprecipitation (IP) and Immunostaining (Immunohistochemistry, IHC) applications.
Suggested dilutions for: Immunoprecipitation (IP): 1-5µg/5µl sepharose
Immunostaining: 0.5-1µg/ml
Store at -20°C.
For research use only.
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Host Mouse
- Clonality Monoclonal
- Application Immunoprecipitation IP Immunocytochemistry ICC
- Form 100µl
- Specificity Specific for Native GFP and its variant from jellyfish Aequorea victoria. Denatured protein lacks antigen determinants
- Species Reactivity NULL
- Preparation Form PBS solution containing 10 mmol/L HEPES and 10 mmol/L sodium azide
- Concentration lot dependent
- Clone Number mFX73
- Isotype IgG2a
- Source Species Mouse
- Shelf Life NULL
- Cross Reactivity Native and variant GFP
- Immunogen GFP partial peptide coupled with BSA
Storage Details
- Pack Description 100µl
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.0015
- Pack Width (m) 0.0015
- Pack Height (m) 0.0022
- Pack Weight (kg) 0.00