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Quantitative determination of sulfidoleukotriene from basophils in blood in response to allergens. BÜHLMANN CAST® ELISA 192 wells

Product code: EK-CAST

Pack Size: 192 wells
CE Verified: CE IVD

Product Information

Mediators of allergy, such as leukotrienes or histamine are produced and/or secreted upon contact of allergen with IgE molecules on specialized cells such as basophils and mast cells. In contrast to histamine, leukotrienes are also synthesized and released in the case of the pseudo-allergies, reactions resembling allergy, but without involvement of any IgE molecules.
BÜHLMANN CAST® ELISA the Cellular Allergen Stimulation Test, can also be considered as an in vitro allergy provocation test. The collected patient's leukocyte blood fraction will be exposed in vitro to the suspected specific allergen. After incubation the cellular supernatant will be tested for the de novo synthesis of sulfidoleukotrienes by a highly specific ELISA.
This test detects IgE-mediated allergies, but also the pseudo-allergies. This latter kind of allergy is best known for Aspirin (ASA) and other non-steroidal anti-inflammatory drugs (NSAIDs) and various other drugs or food additives.

Product Details:
Product Code: EK-CAST
Specimen Type: Blood into EDTA tubes. It is recommended that systemically administered antiallergenic drugs such as antihistamines, corticosteroids or chromoglycic acid (DSCG) should be avoided before sample collection. In order to avoid any interaction with systemically administered drugs BÜHLMANN recommends stopping the administration, if possible, three to seven days before blood sample collection. The exact waiting period depends on different factors, such as pharmacokinetic and bioavailability of substances, duration and regime of administration etc. Patients supposed to suffer from drug hypersensitivity should not be tested for the drug immediately after an adverse reaction. A recovery phase of several weeks after the adverse reaction is recommended. Different studies propose the highest sensitivity of the assay by testing the patient within about half a year after the adverse reaction to drugs. Important: Blood samples from patients going to be tested in vivo like skin test or oral provocation, must be drawn before any exposure to the allergen happens
Sample volume: 200µl
Test Range: 50 - 3200ng/ml
Sensitivity: 19pg/ml
Test type: ELISA
Time to Result: 5.5 hour
CE/IVD marked: Yes
Number of wells: 192 wells
Storage conditions: 2-8C


  • Brand BÜHLMANN
  • Sterile N
  • CE Certified CE IVD
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Shelf Life minimum 6 months
  • Sensitivity 19pg/ml
  • Application quantitative determination of sulfidoleukotrienes (sLT) produced by isolated leukocytes upon contact with specific antigens
  • Specificity NULL
  • Contents 1 x 20ml Dextran solution, 1 vial lyophilised stimulation buffer, 1 vial lyophilised Stimulation control, 2 x 12 x 8 microtitre plates, 6 x plate sealer, 1 x 50ml wash buffer concentrate (x20), 1 x 30ml ELISA buffer, 5 vials lyophilised calibrator, 5 x 2 vials lyophilised control, 1 vial lyophilised blanking reagent, 2 vials lyophilised enzyme label, 1 x 11ml antibody, 1 x 42ml pNPP substrate, 1 x 11ml Stop solution
  • Time Required 5.5 hours

Storage Details

  • Pack Description 192 wells
  • Shipping Condition Ambient
  • Storage Condition Fridge
  • Pack Length (m) 0.22
  • Pack Width (m) 0.16
  • Pack Height (m) 0.11
  • Pack Weight (kg) 0.10

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