Faecal calprotectin Immuno turbidimetric assay reagents fCAL™ turbo BÜHLMANN approx 200 tests to differentiate IBS vs IBD & monitor disease

Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.
Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.
The BÜHLMANN fCAL® turbo is ideal for use in high throughput laboratory environments for screening and monitoring IBD patients and can be used on many open access biochemistry analysers. It is a turbidimetric immunoassay based on the standardisation of the BÜHLMANN fCAL® ELISA:
Code: B-KCAL-RSET (1 of each of R1 and R2 reagents)
Storage conditions: 2-8C - do NOT freeze
Sample Type: Faeces
Sample preparation: CALEX or weighing with 1:500 dilution
Sample volume: 10µl of 1:500 extract
Time to first result: 10 minutes
Assay Range: 20 - 8000µg/g
Number of tests: Approximately 200 (dependent on the platform used)
Assay protocols available:Siemens Advia 1650, Advia 1800, Advia 2400, Advia XPT and Atellica, Roche Cobas 6000 (c501/c502) and 8000 (c701/c702, Abbott Architect and Alinity, Mindray BS200e, Mindray BS380, Beckman AU series and Biosystems BA200
All reagents are ready to use and have an on board stability of 90 days
Calibrators and controls are supplied seperately
Calibration is stable for 60 days
Limit of Quantification: 23.7µg/g
Manufacturers cut-off of 80µg/g = Negative, >160µg/g = Positive