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Faecal calprotectin Immuno turbidimetric assay extraction buffer fCAL™ turbo BÜHLMANN to differentiate IBS vs IBD and monitor disease

Product code: B-CAL-EX12

Pack Size: Each
CE Verified: CE IVD
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Product Information

Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.
Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.
The BÜHLMANN fCAL® turbo is ideal for use in high throughput laboratory environments for screening and monitoring IBD patients and can be used on many open access biochemistry analysers. It is a turbidimetric immunoassay based on the standardisation of the BÜHLMANN fCAL® ELISA:
Code: B-KCAL-EX12 (12 x 125ml)
Sample Type: Faeces
Sample preparation: CALEX or weighing with 1:500 dilution
Sample volume: 10µl of 1:500 extract
Time to first result: 10 minutes
Assay Range: 20 - 8000µg/g
Assay protocols available: Siemens Advia 1650, Advia 1800, Advia 2400, Advia XPT, Roche Cobas 6000 (c501), Abbott Architect, Mindray BS200e, Mindray BS380
All reagents are ready to use and have an on board stability of 60 days
Calibration is stable for 60 days
Analytical Sensitivity: 6µg/g Calprotectin in faecal sample
Limit of Quantification: 11µg/g
Established cut-off of 50µg/g = Negative, >200µg/g = Positive


  • Brand BÜHLMANN
  • Sterile N
  • CE Certified CE IVD
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Source Species NULL
  • CAS Number NULL
  • Application extraction of calprotectin from stool samples
  • Molecular Formula NULL
  • Form NULL
  • Concentration NULL
  • Purity NULL
  • Marketed Grade NULL
  • Molecular Weight NULL
  • Shelf Life 365
  • Species Reactivity NULL
  • Preparation Form Liquid

Storage Details

  • Pack Description Each
  • Shipping Condition Ambient
  • Storage Condition Fridge
  • Pack Length (m) 0.22
  • Pack Width (m) 0.16
  • Pack Height (m) 0.11
  • Pack Weight (kg) 0.10

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