This product is for Professional Use only and not for sale to the general public for home use.
Please log in with a business account to purchase.
Restriction Endonuclease Xho I (high conc.) DNA Cleavage 5000U Nippon Gene Wako
Product code: 319-02161
Pack Size: 5000U
CE Verified: Research Use Only
Contact us
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
Restriction Endonuclease Xho I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Xho I 5000U (High Conc)
Recognition sequence:
5 '...... C ▼ TCGA G ...... 3'
3 '...... G AGCT ▲ C ...... 5'
Source : Xanthomonas holcicola (ATCC 13461)
Activity: 30 - 100 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mmol/l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Xho I 5000U (High Conc)
Recognition sequence:
5 '...... C ▼ TCGA G ...... 3'
3 '...... G AGCT ▲ C ...... 5'
Source : Xanthomonas holcicola (ATCC 13461)
Activity: 30 - 100 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mmol/l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Xanthomonas holcicola (ATCC 13461)
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 30 - 100 units / μl
- Concentration 5000U
- Cut Site 5 '...... C ▼ TCGA G ...... 3'
3 '...... G AGCT ▲ C ...... 5' - Heat Inactivation No
Storage Details
- Pack Description 5000U
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
You may also be interested in
Prev
Next