This product is for Professional Use only and not for sale to the general public for home use.
Please log in with a business account to purchase.
Restriction Endonuclease Sfi I DNA Cleavage 100U Nippon Gene Wako
Product code: 319-01441
Pack Size: 100U
CE Verified: Research Use Only
Contact us
This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
Restriction Endonuclease Sfi I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Sfi I 100U
Recognition sequence:
5 '... GGCCN NNN ▼ NGGCC ...... 3'
3 '... CCGGN ▲ NNN NCCGG ...... 5'
Source : Streptomyces fi mbriatus
Activity: 5-20 units / μl
Reaction Conditions: 50°C in 1x buffer M
Storage buffer:
200 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer M:
500 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Sfi I 100U
Recognition sequence:
5 '... GGCCN NNN ▼ NGGCC ...... 3'
3 '... CCGGN ▲ NNN NCCGG ...... 5'
Source : Streptomyces fi mbriatus
Activity: 5-20 units / μl
Reaction Conditions: 50°C in 1x buffer M
Storage buffer:
200 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer M:
500 mmol/L NaCl
100 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 50°C
- Source Species Streptomyces fi mbriatus
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 50°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 100U
- Cut Site 5 '... GGCCN NNN ▼ NGGCC ...... 3'
3 '... CCGGN ▲ NNN NCCGG ...... 5' - Heat Inactivation No
Storage Details
- Pack Description 100U
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
You may also be interested in
1 ? activePage - 1 : activePage" >Prev
Next