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Restriction Endonuclease Nco I (high conc.) DNA Cleavage 400U Nippon Gene Wako
Product code: 319-03021
Pack Size: 400U
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Nco I produced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Nco I 400U (High Conc)
Recognition sequence:
5 '... C ▼ CATG G ...... 3'
3 '... G GTAC ▲ C ...... 5'
Source : Nocardia corallina (ATCC 19,070)
Activity: 30 - 100 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 60°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Nco I 400U (High Conc)
Recognition sequence:
5 '... C ▼ CATG G ...... 3'
3 '... G GTAC ▲ C ...... 5'
Source : Nocardia corallina (ATCC 19,070)
Activity: 30 - 100 units / μl
Reaction Conditions: 37°C in 1x buffer H
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.2mg / ml BSA
50% Glycerol
Reaction buffer H:
1000 mmol/L NaCl
500 mmol / l Tris-HCl (pH 7.9 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Remaining activity < 5% after 30min at 60°C
Heat inactivation must also take into consideration the DNA denaturation temperature and complete inactivation may not be possible by heat treatment alone, to ensure deactivation phenol treatment is recommended.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Nocardia corallina (ATCC 19,070)
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 30 - 100 units / μl
- Concentration 400U
- Cut Site 5 '... C ▼ CATG G ...... 3'
3 '... G GTAC ▲ C ...... 5' - Heat Inactivation 30 min at 60°C < 5% activity
Storage Details
- Pack Description 400U
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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