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Restriction Endonuclease Hind III DNA Cleavage 5 x 1200U Nippon Gene Wako
Product code: 319-01164
Pack Size: 12000Ux5
CE Verified: Research Use Only
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Product Information
Restriction Endonuclease Hind IIIproduced by Nippon Gene Co Ltd distributed via FUJIFILM Wako Chemical Corporation
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Hind III 5 x 12000U
Recognition sequence:
5 '... A ▼ AGCT T ...... 3'
3 '... T TCGA ▲ A ...... 5'
Source : Haemophilus influenzae Rd
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer B
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.5mg / ml BSA
50% Glycerol
Reaction buffer B :
1000 mmol/L NaCl
100 mmol / l Tris-HCl (pH 8.5 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Restriction endonucleases are enzymes that recognise and cleave specific nucleotide sequences of DNA. This restriction enzyme is supplied with concentrated reaction buffer 10X, please dilute to give a reaction mixture with the necessary conditions for the enzyme reaction.
Hind III 5 x 12000U
Recognition sequence:
5 '... A ▼ AGCT T ...... 3'
3 '... T TCGA ▲ A ...... 5'
Source : Haemophilus influenzae Rd
Activity: 5 - 20 units / μl
Reaction Conditions: 37°C in 1x buffer B
Storage buffer:
50 mmol / l KCl
10 mmol / l Tris-HCl (pH 7.5)
0.1 mmol / l EDTA
1 mmol / l DTT
0.5mg / ml BSA
50% Glycerol
Reaction buffer B :
1000 mmol/L NaCl
100 mmol / l Tris-HCl (pH 8.5 at 25°C)
100 mmol/L MgCl2
10 mmol / l DTT
Heat inactivation: Not inactivated by heat, deactivate by treatment with phenol.
Store at -20°C
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Optimal Temperature 37°C
- Source Species Haemophilus influenzae Rd
- Unit Definition One unit is defined as the amount of enzyme that cleaves 1 µg of Lambda DNA completely in 1 hour at 37°C in 50 µl of reaction mixture.
- Specific Activity 5 - 20 units / μl
- Concentration 12000U
- Cut Site 5 '... A ▼ AGCT T ...... 3'
3 '... T TCGA ▲ A ...... 5' - Heat Inactivation No
Storage Details
- Pack Description 12000Ux5
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.06
- Pack Width (m) 0.06
- Pack Height (m) 0.06
- Pack Weight (kg) 0.00
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