DAPI 4',6-Diamidino-2-phenylindole Solution (1mg/ml) Cell Analysis Dead Cell Stain 1ml Cellstain® Dojindo Laboratories Wako

Cellstain® DAPI Solution 1ml produced by Dojindo Laboratories and distributed in association with FUJIFILM Wako Chemicals Europe GmbH


The Cellstain® range, produced by Dojindo Laboratories is intended for research use to facilitate cell analysis. Cellstain® 4',6-Diamidino-2-phenylindole (DAPI) is an AT-sequence-specific DNA intercalator that attaches to DNA at the minor groove of the double helix like Hoechst dyes. DAPI is not permeable through viable cell membranes, but it passes through disturbed cell membranes to stain the nucleus. DAPI has a high photobleaching tolerance level. DAPI is utilized for the detection of mitochondrial DNA in yeast, chloroplast DNA, virus DNA, micoplasm DNA, and chromosomal DNA. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively. Supplied as 1mg DAPI per 1ml of buffer.

Staining Procedure
Prepare 10-50 µM DAPI solution with PBS or an appropriate buffer.^a)
1. Add DAPI solution with 1/10 of the volume of cell culture medium to the cell culture.^b)
2. Incubate the cell at 37°C for 10-20 min.
3. Wash cells twice with PBS or an appropriate buffer.
4. Observe the cells using a fluorescence microscope with 360 nm excitation and 460 nm emission filters.
a) Since DAPI may be carcinogenic, extreme care is necessary during handling.
b) Or you may replace the culture medium with 1/10 concentration of DAPI buffer solution.
Cellstain® DAPI Solution (1mg/ml) 1ml
Also known 4',6-Diamidino-2-phenylindole, dihydrochloride, aqueous solution Dojindo Laboratories: D523-10
Pack Size: 1mg
Molecular Formula:C₁₆H₁₇Cl₂N₅
MW: 350.25
CAS No: 28718-90-3
Store 0-5°C, protect from light
Ships at ambient temperature

Reference
1. W. Schnedl, et al., DIPI and DAPI: Fluorescence Banding with Only Negligible Fading. Hum Genet. 1977;36:167-172.
2. I. W. Taylor, et al., An Evaluation of DNA Fluochromes, Staining Techniques, and Analysis for Flow Cytometry. I. Unperturebed Cellpopulations. J Histochem Cytochem. 1980;28:1224-1232.
3. F. Otto, et al., A Comparative Study of DAPI, DIPI, and Hoechst 33258 and 33342 as Chromosomal DNA Stains. Stain Technol. 1985;60:7-11.
4. N. Poulin, et al., Quantitative Precision of an Automated Image Cytometric System for the Measurement of DNA Content and Distribution in Cells Labeled with Fluorescent Nucleic Acid Stains. Cytometry. 1994;16:227-235.
5. M. Kawai, et al., Rapid Enumeration of Physiologically Active Bacteria in Purified Water Used in the Pharmaceutical Manufacuturing Process. J Appl Microbiol. 1999;86:496-504.