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Calcein AM Solution Cell Analysis Live Cell viability Stain (1mM in DMSO) 1ml Cellstain® Dojindo Laboratories Wako

Product code: 341-07901

Pack Size: 1ml
CE Verified: Research Use Only
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Product Information

Cellstain® Calcein-AM Solution 1mM (1mg/ml) produced by Dojindo Laboratories and distributed in association with FUJIFILM Wako Chemicals Europe GmbH

The Cellstain® range, produced by Dojindo Laboratories is intended for research use to facilitate cell analysis. Cellstain® Calcein-AM Solution is a live cell stain. Calcein-AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity compared to Calcein. After Calcein-AM permeates into the cytoplasm, it is hydrolyzed by esterases to Calcein, which remains inside the cell. Among other reagents, including BCECF-AM and Carboxy-fluorescein diacetate, Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not inhibit any cellular functions such as proliferation or chemotaxis of lymophocyte. In addition, viability assays using Calcein are reliable and correlate well with the standard 51Cr-release assay. The excitation and emission wavelengths of calcein are 490 nm and 515 nm, respectively

Staining Procedure
1. Dilute 1 mM Calcein-AM solution with PBD to prepare 1-50 µM Calcein-AM solution.a)
2. Add Calcein-AM solution with 1/10 of the volume of cell culture medium to the cell culture. b)
3. Incubate the cell at 37°C for 15-30 min.
4. Wash cells twice with PBS or an appropriate buffer.
5. Observe the cells under a fluorescence microscope with 490 nm excitation and 515 nm emission filters.
a) If the Calcein-AM has difficulty loading into cells, use a detergent such as Pluronic F127.
b) Or you may replace the culture medium with 1/10 concentration of Calcein-AM buffer solution.
Cellstain® Calcein-AM Solution 1mM (1mg/ml in DMSO)
Also known as 3',6'-Di(O-acetyl)-4',5'-bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein tetraacetoxymethyl ester, DMSO solution
Dojindo Laboratories: C396-10
Pack Size: 1ml
Molecular Formula:C48H46N2O23
MW: 994.86
CAS No: 148504-34-1 (Calcein-AM)
Store -20°C, protect from light
Ships with ice packs


1. K. McGinnes, et al., A Fluorescence NK Assay Using Flow Cytometry. J Immunol Methods. 1986;86:7-15.
2. S. J. Morris, Real-time Multi-wavelength Fluorescence Imaging of Living Cells. BioTechniques. 1990;8:296-308.
3. S. A. Weston, et al., New Fluorescent Dyes for Lymphocyte Migration Studies Analysis by Flow Cytometry and Fluorescent Microscopy. J Immunol Methods. 1990;133:87-97.
4. S. A. Weston, et al., Calcein: a Novel Marker for Lymphocytes Which Enter Lymph Nodes. Cytometry. 1992;13:739-749.
5. X. M. Wang, et al., A New Microcellular Cytotoxicity Test Based on Calcein AM Release. Hum Immunol. 1993;37:264-270.
6. N. G. Papadopoulos, et al., An Improved Fluorescence Assay for the Determination of Lymphocyte-Mediated Cytotoxicity Using Flow Cytometry. J Immunol Methods. 1994;177:101-111.
7. H. Ohata, et al., Confocal Imaging Analysis of ATP-Induced Ca2+ Response in Individual Endothelial Cells of the Artery in Situ. Am J Physiol. 1997;272:C1980-C1987.


  • Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
  • Sterile N
  • CE Certified Research Use Only
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Contents Cellstain® Calcein-AM 1mM (1mg/ml in DMSO)

Storage Details

  • Pack Description 1ml
  • Shipping Condition Blue Ice
  • Storage Condition -20
  • Pack Length (m) 0.035
  • Pack Width (m) 0.035
  • Pack Height (m) 0.08
  • Pack Weight (kg) 0.00

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