Beta Amyloid 1-40 ELISA kit Human plasma tissue culture medium tissue homogenate CSF Wako

Human Beta Amyloid (1-40) ELISA produced by FUJIFILM Wako Chemical Corporation

Beta Amyloid protein is a major component of senile plaques. Beta Amyloid can exist as a 40 or 42 amino acid peptide which is produce by cleavage of the Amyloid Precursor protein by beta- or gamma-Secretase. The 42 amino acid form of Beta Amyloid is more prone to aggregate than the 40 a.a. form and initial beta amyloid deposition begins with beta Amyloid 42. Plaques that are positive for beta amyloid 42 but negative for beta amyloid 40 may represent early-stage diffuse type senile plaques, beta amyloid 40 positive plaques may appear in the advanced stage.
Reduced beta amyloid 42 in CSF and ratios of beta amyloid 42 to total beta amyloid have been described as diagnostic markers of Alzheimer's disease. This ELISA uses monoclonal antibody BAN50 which binds the N-terminal of Beta Amyloid peptides (1-16) and monoclonal antibody BA27 which specifically detect the C-terminal portion of Beta Amyloid 40. Therefore the ELISA detects Beta Amyloid (1-40).
Suitable for use with human plasma, tissue culture medium, tissue homogenate and CSF.

Kit contents:
Antibody coated Microtiter wells (Anti-Human Aβ(1-16) MAb Clone BAN50) 12x8 well strips
Human Aβ (1-40) Standard, 100pmol/L 2x2ml
Standard diluent 30ml
Wash solution concentrate (20X) 50ml
HRP-conjugated antibody (Anti-Human Aβ(1-40) MAb Clone BA27 Fab’-HRP 12ml
TMB Solution 12ml
Stop Solution 12ml
Plate seal 3 sheets

Sample volume : 100µl
Incubation times: Overnight at 4C, 1 h at 4C, 30 min room temperature
Dynamic range: 1.0-100 pmol/L
Cross-reactivity with Human Aβ(1-42) and Aβ(1-43) ≤0.1%
Cross-reactivity with Rat (mouse) Aβ(1-40) and Aβ(1-42) ≤0.4%

Store at 2-4C.
For Research Use Only.