Cryptococcus Lateral Flow Assay Test for serum, plasma, whole blood and cerebral spinal fluid. IMMY 50 tests

The CrAg Lateral Flow Assay is an immunochromatographic test system for the qualitative or semi-quantitative detection of the capsular polysaccharide antigens of Cryptococcus species complex (Cryptococcus neoformans and Cryptococcus gattii) in serum, plasma, whole blood and cerebral spinal fluid (CSF).
The CrAg Lateral Flow Assay is a dipstick sandwich immunochromatographic assay. Specimens and specimen diluent are added into an appropriate reservoir, such as a test tube, and the lateral flow device is placed into the reservoir. The test uses specimen wicking to capture gold conjugated, anti-CrAg monoclonal antibodies and gold conjugated control antibodies deposited on the test membrane.CrAg present in the specimen binds to the gold-conjugated, anti-CrAg antibodies. The gold labelled antibody-antigen complex continues to wick up the membrane where it will interact with the test line, which has immobilised anti-CrAg monoclonal antibodies. The antibody-antigen complex forms a sandwich at the test line causing a visible line to appear. With proper flow and reagent reactivity, the wicking of any specimen, positive or negative, will cause the gold-conjugated control antibody to move to the control line. Immobilised antibodies at the control line will bind to the gold-conjugated control antibody and form a visible control line. Positive test results create two lines (test and control). Negative test results form only one line (control). If a control line fails to develop then the test is not valid.