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Serum Calprotectin (MRP8/14) ELISA for disease monitoring BÜHLMANN 96 well plate

Product code: EK-MRP8/14

Pack Size: 96 Wells
CE Verified: CE IVD
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Product Information

Plasma MRP8/14 (calprotectin) levels are increased in various inflammatory conditions and provides an early and sensitive marker of acute coronary syndromes, acute kidney allograft rejection and rheumatoid arthritis. The diagnostic value and advantage of MRP8/14 detection over other disease markers is that it is stored in the cell and released immediately in response to local inflammation. In contrast, other markers may be generated by downstream pathways or need to be synthesized de novo. The BÜHLMANN MRP8/14 assays allow for precise and sensitive measurement of this important diagnostic marker.

Product Details:
Code: EK-MRP8/14
Sample Type: Serum or plasma. EDTA Plasma: Collect blood into EDTA tubes. Centrifuge at 3000 x g for 5 minutes at room temperature within 4 hours after drawing and collect the plasma. Do not heat-inactivate samples. Samples may be stored at 2-8°C for up to 30 days. If samples have to be stored for a longer period, they are stable at ≤-20°C for at least 6 months. Serum: Collect blood into plain tubes, avoid haemolysis, mix by gentle inverting sample tubes several times and leave to clot for 45 minutes at room temperature (18-28°C) protected from light. Centrifuge at 1800 x g for 15 minutes at room temperature (18-28°C) and collect the serum immediately. Do not heat-inactivate samples. Samples may be stored at 2-8°C for up to 30 days. If samples have to be stored for a longer period, they are stable at ≤-20°C for at least 6 months. Lipemic, hemolytic and icteric samples should not be used in this assay. Lipemic samples can be avoided by asking patients to fast for at least 12 hours prior to drawing the sample. Note: MRP8/14 measurement in plasma from heparinized or citrated blood samples is possible. Be aware that normal values are slightly effected by the sample type used.
Test Time: 75 minutes
Assay Range: 0.4 - 24µg/ml
Sensitivity: < 0.4µg/ml
Number of wells: 96
Assay protocols: DS2 and Triturus
All reagents are ready to use (except the wash buffer)
Calibrators and controls are included


  • Brand BÜHLMANN
  • Sterile N
  • CE Certified CE IVD
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Shelf Life minimum 6 months
  • Sensitivity 0.4µg/g
  • Application quantification of serum calprotectin
  • Specificity cross reactivity < 0.1%
  • Contents 12x8 wells pre-coated with anti-calprotectin mAb;3xplate seals;1x100ml wash buffer concentrate (10x)(dilute before use);1 x100ml incubation buffer; Calibrators A-E 5x1ml; Controls low and high 2x1ml; 1x11ml Anti-Calprotectin Ab conjugated to HRP;1x11ml TMB substrate; 1x11ml Stop Solution
  • Time Required 75min

Storage Details

  • Pack Description 96 Wells
  • Shipping Condition Ambient
  • Storage Condition Fridge
  • Pack Length (m) 0.22
  • Pack Width (m) 0.16
  • Pack Height (m) 0.11
  • Pack Weight (kg) 0.10

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