Follicle Stimulating Hormone (FSH) ELISA for serum or plasma 1-100 mIU/ml Dialab

Human follicle-stimulating hormone (FSH, follitropin) is a glycoprotein produced and secreted by the basophilic cells of the anterior lobe of the pituitary gland. Secretion of FSH is stimulated by gonadotrophin-releasing hormone (GnRH). Gonodal steroids like progesterone, oestrogens and androgens, exert both positive and negative feedback on FSH function. Pulse-like secretion of FSH is more pronounced in women than in men, In women, FSH levels vary during the menstrual cycle. FSH stimulates maturation of ovarian follicles at the beginning of the cycle. Ovulation is preceded by a surge in FSH (mid-cycle phase). Base levels are slightly higher at the beginning of the cycle (follicular phase) than at the end of the cycle (luteal phase). In postmenopausal women, FSH levels are significantly increased because of lack of negative feedback from ovarian steroids. In me, FSH and LH maintain spermatogenesis in the tested. FSH levels in pre-pubertal children are low. FSH determination is important in diagnosis in women with disturbances of the menstrual cycle,primary and secondary amenorrhea, hirsutism or virilism. FSH is a good indicator of onset of menopause. In men, determination of FSH is useful in the diagnosis of infertility, hypogonadism, gynaecomastia and tumours. In children, assessment of FSH is important in investigating delayed or precocious puberty.

Male: 2.0-14.0 mIU/mL
Female:
Follicular phase: 2.0-10.0 mIU/mL
Ovulatory phase: 6.0-24.0 mIU/mL
Luteal phase: 1.5-8.0 mIU/mL
Post-menopausal: 17.0-95.0 mIU/mL
Pregnant female: 0.0-11.6 mIU/mL

This assay is intended for the quantitative determination of FSH in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 42 patient tests. FSH in the sample is bound to the antibody that coats the 96 well plate. The antigen-antibody complex is then reacted with anti-FSH horseradish peroxidase (HRP) conjugate resulting in the FSH antigen being sandwiched between the solid phase antibody and the enzyme conjugate. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is proportional to the concentration of FSH in the sample.

Kit contents:
Antibody coated Microtiter wells (Anti-FSH, monoclonal): 12x8 well strips
Calibrator set (0.0 mIU/ml x 1ml; 5, 12, 40, 100 mIU/ml x 0.4ml)
Enzyme Conjugate (Anti-FSH-HRP): 1x11ml
Substrate Solution (H₂O₂-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml

Sample volume: 50µl human serum or plasma to be run in duplicate
Incubation time: 80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Calibration range: 1-100 mIU/mL
Sensitivity: 1.00 mIU/mL
Specificity: hFSH 100.00%, hLH < 1.0%, hCG < 1.0%, PRL < 1.0%
Store at 2-8°C.