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Prolactin ELISA for serum or plasma 1-200 ng/ml Dialab
Product code: Z05303
Pack Size: 96 Wells
CE Verified: CE IVD
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
Prolactin (PRL) is a polypeptide hormone secreted by the anterior pituitary gland. Release of prolactin is pulsatory, and regulated by hypothalamic releasing and inhibitory factors. High prolactin levels are observed during pregnancy and the postpartum-lactation period. Other physiological states associated with high prolactin levels include physical and emotional stress, sleep and hypoglycaemia. Hyperprolactinaemia can cause suppression of gonadal function. Prolactin measurement therefor form an essential part of investigations fo infertility. In women, symptoms that may accompany hyperprolactinaemia included prolactin secreting macro- and microadenomas hypothyroidism and renal failure.
Male: 4.0-12.0 ng/ml
Female:
Menstrual cycle: 1.2-22.0 ng/ml
Menopausal: 0.7-17.0 ng/ml
This assay is intended for the quantitative determination of PRL in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 42 patient tests. PRL in the sample is bound to the monoclonal antibody that is immobilised on the 96 well plate. The antigen-antibody complex is then reacted with anti-PRL horseradish peroxidase (HRP) conjugate resulting in the PRL antigen being sandwiched between the solid phase antibody and the enzyme conjugate. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is proportional to the concentration of PRL in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-PRL, monoclonal): 12x8 well strips
Calibrator set (0.0 ng/ml x 1ml; 5, 20, 75, 200 ng/ml x 0.15ml)
Enzyme Conjugate (Anti-PRL-HRP): 1x11ml
Substrate Solution (H2O2-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml
Sample volume: 10µl human serum or plasma to be run in duplicate
Incubation time: 80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Calibration range: 0-200 ng/mL
Sensitivity: 1ng/ml
Specificity: hPRL 100.00%, hLH < 1.0%, hCG < 1.0%, FSH < 1.0%
Store at 2-8°C.
Male: 4.0-12.0 ng/ml
Female:
Menstrual cycle: 1.2-22.0 ng/ml
Menopausal: 0.7-17.0 ng/ml
This assay is intended for the quantitative determination of PRL in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 42 patient tests. PRL in the sample is bound to the monoclonal antibody that is immobilised on the 96 well plate. The antigen-antibody complex is then reacted with anti-PRL horseradish peroxidase (HRP) conjugate resulting in the PRL antigen being sandwiched between the solid phase antibody and the enzyme conjugate. Then the enzyme HRP in the bound reaction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is proportional to the concentration of PRL in the sample.
Kit contents:
Antibody coated Microtiter wells (Anti-PRL, monoclonal): 12x8 well strips
Calibrator set (0.0 ng/ml x 1ml; 5, 20, 75, 200 ng/ml x 0.15ml)
Enzyme Conjugate (Anti-PRL-HRP): 1x11ml
Substrate Solution (H2O2-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml
Sample volume: 10µl human serum or plasma to be run in duplicate
Incubation time: 80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Calibration range: 0-200 ng/mL
Sensitivity: 1ng/ml
Specificity: hPRL 100.00%, hLH < 1.0%, hCG < 1.0%, FSH < 1.0%
Store at 2-8°C.
Specification
- Brand Dialab
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life NULL
- Sensitivity 1ng/ml
- Application This assay is intended for the quantitative determination of PRL in human serum or plasma for diagnostic use.
- Specificity hPRL 100.00%, hLH < 1.0%, hCG < 1.0%, FSH < 1.0%
- Contents Antibody coated Microtiter wells (Anti-PRL, monoclonal): 12x8 well strips, Calibrator set (0.0 mIU/ml x 1ml; 5, 20, 75, 200 ng/ml x 0.15ml), Enzyme Conjugate (Anti-PRL-HRP): 1x11ml, Substrate Solution (H2O2-TMB 0.25g/L): 1x11ml
Stop Solution (Sulphuric Acid 0.15mol/L): 1x11ml - Time Required Incubation time:80 minutes (60 min at room temperature, 20 min in the dark at room temperature)
Storage Details
- Pack Description 96 Wells
- Shipping Condition Ambient
- Storage Condition Fridge
- Pack Length (m) 0.12
- Pack Width (m) 0.12
- Pack Height (m) 0.1
- Pack Weight (kg) 0.00