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Faecal Calprotectin ELISA BÜHLMANN fCAL™ without Buffer for differentiating IBS vs IBD and disease monitoring. EK-CAL2 2x96 Wells

Product code: EK-CAL2-WEX

Pack Size: 2 x 96Well
CE Verified: CE IVD

Product Information

Calprotectin is a heterodimeric calcium binding protein of the S100 family predominantly found in the cytosolic fraction of neutrophils. It is also found to some extent in monocytes and activated macrophages. Plasma calprotectin (also known as MRP8/14) levels are increased in various inflammatory conditions. The concentration of calprotectin is greater in faeces than in plasma and significantly increased levels are found in patients with bowel inflammation. Measurement of faecal Calprotectin is considered a reliable indicator of inflammation and numerous studies show that while faecal Calprotectin concentrations are significantly elevated in patients with Inflammatory Bowel Disease (IBD), patients suffering from Irritable Bowel Syndrome (IBS) do not have increased Calprotectin levels. Therefore, faecal calprotectin measurement can help aid differential diagnosis between IBD and IBS.
Increased levels of faecal calprotectin are shown to correlate well with both endoscopic and histological assessment of disease activity and so Calprotectin assays can also be used to monitor patient responses to therapy and help predict relapse.
The BÜHLMANN fCAL® ELISA is ideal for use in high through put laboratory environments for screening and monitoring IBD patients. The EK-CAL2-WEX does not contain any buffer and is designed for use with the CALEX Cap extraction device (B-CALEX-C50 or B-CALEX-C200) which can be used directly on the ELISA processor:
Code: EK-CAL2-WEX
Sample Type: Faeces
Test Time: 75 minutes
Assay Range: 30 - 1800µg/g or 10 - 600µg/g (Different test protocol to achieve the higher or lower range)
Number of wells: 2 x 96 Each plate is individually sealed (up to 80 samples in duplicate)
Assay protocols: DS2 and Triturus
All reagents are ready to use (except the wash buffer)
No Extraction Buffer included
Calibrators and controls are included
Analytical Sensitivity: < 10µg/g Calprotectin in faecal sample
Manufacturers cut-off of < 80µg/g = Negative >160µg/g = Positive

Specification

  • Brand BÜHLMANN
  • Sterile N
  • CE Certified CE IVD
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • Shelf Life minimum 6 months
  • Sensitivity 10µg/g
  • Application quantification of faecal calprotectin for differentiation between IBD and IBS; patient monitoring mucosal healing
  • Specificity cross reactivity < 0.1%
  • Contents 2x12x8 wells pre-coated with anti-calprotectin mAb;6xplate seals;2x100ml wash buffer concentrate (10x)(dilute before use);3 x125ml incubation buffer; Calibrators A-E 5x1ml; Controls low and high 2x1ml; 2x12ml Anti-Calprotectin Ab conjugated to HRP;2x12ml TMB substrate; 2x12ml Stop Solution
  • Time Required 75min

Storage Details

  • Pack Description 2 x 96Well
  • Shipping Condition Ambient
  • Storage Condition Fridge
  • Pack Length (m) 0.22
  • Pack Width (m) 0.16
  • Pack Height (m) 0.11
  • Pack Weight (kg) 2.00

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