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α and β Amanitin ELISA for human urine, serum and plasma BÜHLMANN 96 wells for management of suspected mushroom poisoning
Product code: EK-AM1
Pack Size: 96 Wells
CE Verified: CE IVD
Product Information
α Amanitin is the major toxin of the extremely poisonous toadstools, Amanita phalloides (death cap), A. verna (white death cap), A. virosa (destroying angel) and other Amanita species. These mushrooms may also contain other amatoxins such as β and γ Amanitin as well as phallotoxins. Amatoxins are also found in Galerina arginata
(marginate pholiota), G. autumnalis, G. sulciceps and other Galerina and Lepiota species. A. phalloides, A. verna and A. virosa account for over 90 % of clinically relevant mushroom intoxication cases in Europe & Northern America. Amanita species also occur in Asia and Australia.
Amatoxins infiltrate hepatocytes in which their primary action is to inhibit nucleoplasmic RNA polymerase II (which in turn interferes with mRNA synthesis). This results in the arrest of protein synthesis and cellular necrosis ultimately leading to severe acute hepatitis. Amanitin also acts as a direct nephrotoxin. The symptoms of phallatoxic Amanita species poisoning are of delayed onset type. Patients may therefore not primarily associate their symptoms with the ingestion of the wild mushroom. Once symptoms appear, amatoxins can be detected in urine. The detection of α Amanitin (100 %) and γ Amanitin (90 %) in urine by means of the BÜHLMANN Amanitin ELISA confirms Amanita Intoxication. Amatoxins are detected in urine samples within 6-60 hours after ingestion, i.e. the time by which clinical symptoms occur.
From internal evaluation data and clinical toxicological study, capability characteristics may be outlined as follows:
The Amanitin ELISA test is an additional tool in an entire diagnostic workup.
A negative result does not exclude a possible Amanitin intoxication.
Sample collection must be within 36hours.*
The Amanitin ELISA test detects α Amanitin (100%) and γ Amanitin (90%).
There is no cross-reactivity to β Amanitin.
Product Details:
Specimen Type: Serum, plasma or urine. Samples should be kept refrigerated. Aliquots may be stored for up to 7 days at 2-8°C or for at least 6 months frozen at –20 °C. To reach highest sensitivity, urinary sample collection must be within 36 hours after mushroom ingestion
Sample volume: 40µl
Test type: Quantitative ELISA
Test Range: 10 - 100ng/ml
Sensitivity: 0.2ng/ml
Time to Result: 1 hour
CE/IVD marked: Yes
Number of wells: 96 wells
Storage conditions: 2-8C
(marginate pholiota), G. autumnalis, G. sulciceps and other Galerina and Lepiota species. A. phalloides, A. verna and A. virosa account for over 90 % of clinically relevant mushroom intoxication cases in Europe & Northern America. Amanita species also occur in Asia and Australia.
Amatoxins infiltrate hepatocytes in which their primary action is to inhibit nucleoplasmic RNA polymerase II (which in turn interferes with mRNA synthesis). This results in the arrest of protein synthesis and cellular necrosis ultimately leading to severe acute hepatitis. Amanitin also acts as a direct nephrotoxin. The symptoms of phallatoxic Amanita species poisoning are of delayed onset type. Patients may therefore not primarily associate their symptoms with the ingestion of the wild mushroom. Once symptoms appear, amatoxins can be detected in urine. The detection of α Amanitin (100 %) and γ Amanitin (90 %) in urine by means of the BÜHLMANN Amanitin ELISA confirms Amanita Intoxication. Amatoxins are detected in urine samples within 6-60 hours after ingestion, i.e. the time by which clinical symptoms occur.
From internal evaluation data and clinical toxicological study, capability characteristics may be outlined as follows:
The Amanitin ELISA test is an additional tool in an entire diagnostic workup.
A negative result does not exclude a possible Amanitin intoxication.
Sample collection must be within 36hours.*
The Amanitin ELISA test detects α Amanitin (100%) and γ Amanitin (90%).
There is no cross-reactivity to β Amanitin.
Product Details:
Specimen Type: Serum, plasma or urine. Samples should be kept refrigerated. Aliquots may be stored for up to 7 days at 2-8°C or for at least 6 months frozen at –20 °C. To reach highest sensitivity, urinary sample collection must be within 36 hours after mushroom ingestion
Sample volume: 40µl
Test type: Quantitative ELISA
Test Range: 10 - 100ng/ml
Sensitivity: 0.2ng/ml
Time to Result: 1 hour
CE/IVD marked: Yes
Number of wells: 96 wells
Storage conditions: 2-8C
Specification
- Brand BÜHLMANN
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life minimum 6 months
- Sensitivity 0.2ng/ml
- Application quantitative in vitro diagnostic determination of α and γ Amanitin present in human urine, serum and plasma.
- Specificity The following cross-reactions of the polyclonal rabbit anti- α Amanitin antibody with different Amatoxins and Phallotoxins have been determined at 50% binding.
α Amanitin : 100.0 % ε Amanitin: 0.1 %
β Amanitin : 0.1 % Phalloidin: not detecta - Contents 12 x 8 wells microtitre plate; 3 x plate seals; 1 x 100ml wash buffer concentrate (10x dilute before use); 1 x 100ml incubation buffer; 1 x vial Blanking reagent; 5 vials Calibrators; 2 vials Controls; 1 vial Biotin conjugate; 1 x 11 ml Enzyme label; 1 x 11ml TMB substrate; 1 x 11ml Stop Solution
- Time Required 1 hour
Storage Details
- Pack Description 96 Wells
- Shipping Condition Ambient
- Storage Condition Fridge
- Pack Length (m) 0.22
- Pack Width (m) 0.16
- Pack Height (m) 0.11
- Pack Weight (kg) 0.50