Lactate enzymatic colorimetric assay plasma cerebrospinal fluid 0.1-210mg/dL Sentinel Diagnostics

Lactate enzymatic colorimetric method for use with plasma and cerebrospinal fluid manufactured by Sentinel Diagnostics

Lactate is produced by anaerobic metabolism of glucose, mainly in skeletal muscle. Aerobic energy production is the body's preferred process whereby oxygen and glucose are converted to ATP inside the mitochondria. However, when cellular oxygen levels are low or the mitochondria are not working properly the body must resort to the less efficient process of anaerobic metabolism. During anaerobic energy the rate of lactate production is greater than the rate at which the liver can break it down, which results in increased lactate in the blood - hyperlactataemia which can progress to lactic acidosis which disrupts acid/base balance
Lactate determination is often performed in conjunction with pyruvate, which may be useful id discovering lactic acidosis due to reduced tissue oxygenation or enzymatic deficiencies.

During the test lactate in the sample is oxidised by lactate oxidase to give pyruvate and hydrogen peroxide. In the presence of peroxidase (POD) the hydrogen peroxide subsequently reacts with TOOS to form a coloured compound. The colour intensity as measured at 540nm is proportional to the amount of lactate in the sample.

The Lactate Dry-Fast kit is suitable for use with plasma or cerebrospinal fluid. The lactate reagent is supplied as 5 vials of lyophilised reagent and 5 bottles of diluent. Each vial is reconstituted to give 10 ml of working reagent that is stable for up to 30 days when stored at 2-8C.

The recommended calibrator for this assay is the Clinical Chemistry Calibrator 16550. The Lactate assay has a measuring range of 0.1-210 md/dL and is suitable for manual and automated methods. The recommended QCs for this assay are the Clinical Chemistry Controls 16150 and 16250.