Alkaline Phosphatase ALP Assay enzymatic colorimetric DGKC method serum plasma 6.0-700.0 U/L dual liquid reagent system Sentinel Diagnostics

Alkaline Phosphatase (ALP) Assay manufactured by Sentinel Diagnostics



Alkaline Phosphatase (ALP) is an enzyme found in the liver and biliary tract. Enzyme levels are dependent upon age and sex and increase during bone growth in childhood and in pregnancy. Pathological increases in ALP activity is observed in hepatitis, cirrhosis, malignant tumours and bone diseases. ALP may be of interest to those investigating reproduction, child development, liver disease, bone disease or cancer.

ALP liquid can be used to measure alkaline phosphatase in serum or plasma (heparin or EDTA) samples. This is a enzymatic colorimetric method of determination. ALP catalyses the hydrolysis of p-nitrophenylphosphate in an alkaline medium to give p-nitrophenol and phosphate. By measuring the variation of absorbance of p-nitrophenol over a defined interval of time it is possible to calculate ALP activity in the sample. Analytical range 6-700 U/L. Assay standardised in accordance with DGKC (Deutsche Gesellschaft Fur Klinische Chemie).
ALP Liquid ready-to-use reagents have on-board stability of 30 days with a calibration interval of 7 days. Each kit provides R1:4x20ml and R2:1x20ml. Use with Clinical Chemistry Calibrator (16550) and Clinical Chemistry Controls, level 1 & 2 (16150, 16250). ALP liquid is suitable for both manual and automated methods of determination. A manual method utilising a spectrophotometer to read at 415nm with a path length of 1cm is described in the instructions for use accompanying these reagents. Applications for some popular analysers are also available - please enquire.