Anti-D Alpha (LDM1 clone) for RhD typing manual tube method 10 x 10 ml kit size ALBAclone® Monoclonal Rh D typing sera Quotient

The main component of this reagent is derived from the in vitro culture of the human/mouse heterohybridoma LDM1
which secretes IgM anti-D. The formulation also contains EDTA and 1g/l sodium azide.
The volume delivered by the reagent dropper bottle is approximately 40μl. Bearing this in mind, care should be taken to ensure that appropriate serum: cell ratios are maintained in all test systems.
This reagent has been standardised for use by the techniques described below and therefore its suitability for use in other
techniques cannot be guaranteed.
Tube Technique - Immediate Spin
. Add 1 volume of blood grouping reagent to a test tube.
. Add 1 volume of red cells suspended to 2-3% in PBS pH 7.0 0.2 or 1.5-2% in LISS.
. Mix the test well.
. Centrifuge at 1000g for 10 seconds or at a suitable alternative g force and time.
. Gently shake the tube to dislodge the cell button from the bottom and observe macroscopically for agglutination.
Tube Technique - LISS
. Add 1 volume of blood grouping reagent to a test tube.
. Add 1 volume of red cells suspended to 1.5 - 2% in LISS.
. Mix the test well and incubate for 15 minutes at 37°C.
. Centrifuge at 1000g for 10 seconds or at a suitable alternative g force and time.
. Gently shake the tube to dislodge the cell button from the bottom and observe macroscopically for agglutination.
Slide Technique
. Add 1 volume of blood grouping reagent to an appropriately prepared area of a glass slide e.g. a wax pencil oval.
. Add 1 volume of red cells suspended to 30-45% in PBS pH 7.0  0.2 or in group homologous plasma/serum.
. Mix well by rocking the slide for approximately 30 seconds and incubate the test for 5 minutes at room temperature
with occasional mixing.
. Observe macroscopically for agglutination. This may be facilitated by reading over a diffuse light source.