Creatinine Liquid kinetic CREA assay Jaffe serum plasma urine 0.05-15.0mg/dL dual reagent system Sentinel Diagnostics

Creatinine (CREA) Liquid kinetic modified Jaffé assay manufactured by Sentinel Diagnostics.

Creatinine is a waste product produced in muscles by the breakdown of creatine. Creatine is part of the cycle that produces energy need to contract muscles. Both creatine and creatinine are produces at a relatively constant rate. Almost all creatinine removed from the blood by glomerula filtration in the kidney and excreted. Therefore measurement of creatinine can be used to assess kidney function and assist in the diagnosis and monitoring of renal disease. Because creatinine is related to size and muscle mass age, weight and gender must be taken into consideration when interpreting results.

Sentinel Diagnostic’s Creatinine liquid uses a modified Jaffé method, in the alkaline solution creatinine in the sample forms a coloured complex with picric acid. The intensity of colour as measure at 505nm is proportional to the concentration of creatinine in the sample. The Creatinine Liquid assay is standardised in accordance with NIST (National Institute of Standards and Technology) and can be used to determine creatinine in serum, plasma (heparin or EDTA) or urine. The Creatine Liquid assay range is 0.05-15mg/dL. The two liquid reagents (Reagent 1: 3x65ml; Reagent 2: 3x65ml) are ready-to-use liquid and are suitable for manual methods of determination as detailed on the accompanying pack insert and can also be automated.

Reagents are stored at 15-30C and the on board stability is 30 days with a calibration interval of 14 days. The assay should be calibrated with the Clinical Chemistry Calibrator 16550 and the recommended QCs for this test are Clinical Chemistry Controls 16150 and 16250.