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Ampicillin allergen for use with BÜHLMANN CAST® ELISA and Flow CAST® assays 4 stimulations
Product code: BAG2-C203
Pack Size: 4 Stims
CE Verified: CE IVD
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This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.
Product Information
BÜHLMANN CAST® Allergens are optimized for in vitro Basophil Activation using BÜHLMANN CAST® assays: CAST® ELISA (EK-CAST) and Flow CAST® (FK-CCR).
CAST® drug allergens are delivered lyophilized. Before usage in CAST® assays, they are to be reconstituted with test specific Stimulation Buffer: CAST® ELISA: B-CAST-STB and Flow CAST®: B-CCR-STB.
Add 250μl Stimulation Buffer into the vial and vortex until the lyophilized material has completely dissolved. For some allergens an additional dilution with Stimulation Buffer is recommended.
Remark: Some individuals react positive either at high or at low allergen concentrations, whereas others react positive over a broad range of concentration.
Storage and Stability: Unopened CAST® Allergens are to be stored at the temperature specified on the label until to the expiration date.
Important: Reconstituted or diluted allergens must not be stored and used again!
Optimal time period for stimulation tests: For optimal results, CAST® assays should be run between 3 and 12 weeks after an allergic reaction to the presumed allergen, although specific basophil reaction is often persistent over a longer time period. Blood samples must be drawn for CAST® assays before carrying out a skin test or in vivo provocation with the presumed allergen as in vivo exposure to an allergen can cause an activation of the leucocytes.
Limitations: A negative CAST® assay result for a specific allergen does not exclude potential (even serious) clinical symptoms. Individuals who developed „allergic“ reactions towards protein or drug allergens in the past and whose CAST® assay was negative, should thus be verified with additional methods like the in vivo provocation or skin test before a drug is administered or a patient is exposed to the allergen.
Concentrations and cut-off: Concentrations and cut-off of these allergen groups were optimized regarding maximum specificity und sensitivity. Concentrations to be used in the test and cut-off of individual allergens are reported in the specifications of the allergens. For Flow CAST®, BÜHLMANN recommends the application of the stimulation index (SI) as an additional diagnostic criterion. The SI is defined as the ratio of allergen specific basophil activation and background activation.
Remarks: In order to apply allergen specific cut-offs, QC criteria published in the CAST® instructions must be fulfilled. Cut-offs should serve only as recommendations. Clinically defined cut-offs should be established by each laboratory or through clinical studies.
Product Details:
Product code: BAG2 - C203
Content per vial: 2.5mg
Additional dilution: 1:5
Concentration in stim for CAST ELISA: 2mg/ml
Cut off for CAST ELISA: ≥ 70pg/ml
Concentration in stim for Flow CAST: 2.27mg/ml
Cut off for Flow CAST: ≥ 5% CD63; SI ≥ 2
CAST® drug allergens are delivered lyophilized. Before usage in CAST® assays, they are to be reconstituted with test specific Stimulation Buffer: CAST® ELISA: B-CAST-STB and Flow CAST®: B-CCR-STB.
Add 250μl Stimulation Buffer into the vial and vortex until the lyophilized material has completely dissolved. For some allergens an additional dilution with Stimulation Buffer is recommended.
Remark: Some individuals react positive either at high or at low allergen concentrations, whereas others react positive over a broad range of concentration.
Storage and Stability: Unopened CAST® Allergens are to be stored at the temperature specified on the label until to the expiration date.
Important: Reconstituted or diluted allergens must not be stored and used again!
Optimal time period for stimulation tests: For optimal results, CAST® assays should be run between 3 and 12 weeks after an allergic reaction to the presumed allergen, although specific basophil reaction is often persistent over a longer time period. Blood samples must be drawn for CAST® assays before carrying out a skin test or in vivo provocation with the presumed allergen as in vivo exposure to an allergen can cause an activation of the leucocytes.
Limitations: A negative CAST® assay result for a specific allergen does not exclude potential (even serious) clinical symptoms. Individuals who developed „allergic“ reactions towards protein or drug allergens in the past and whose CAST® assay was negative, should thus be verified with additional methods like the in vivo provocation or skin test before a drug is administered or a patient is exposed to the allergen.
Concentrations and cut-off: Concentrations and cut-off of these allergen groups were optimized regarding maximum specificity und sensitivity. Concentrations to be used in the test and cut-off of individual allergens are reported in the specifications of the allergens. For Flow CAST®, BÜHLMANN recommends the application of the stimulation index (SI) as an additional diagnostic criterion. The SI is defined as the ratio of allergen specific basophil activation and background activation.
Remarks: In order to apply allergen specific cut-offs, QC criteria published in the CAST® instructions must be fulfilled. Cut-offs should serve only as recommendations. Clinically defined cut-offs should be established by each laboratory or through clinical studies.
Product Details:
Product code: BAG2 - C203
Content per vial: 2.5mg
Additional dilution: 1:5
Concentration in stim for CAST ELISA: 2mg/ml
Cut off for CAST ELISA: ≥ 70pg/ml
Concentration in stim for Flow CAST: 2.27mg/ml
Cut off for Flow CAST: ≥ 5% CD63; SI ≥ 2
Specification
- Brand BÜHLMANN
- Sterile N
- CE Certified CE IVD
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Shelf Life 3 years
- Host NULL
- Purity NULL
- Application allergy testing
- Form NULL
- Specificity NULL
- Species Reactivity NULL
- Type NULL
- Concentration 2.5mg
- Marketed Grade NULL
- Preparation Form lyophilised
Storage Details
- Pack Description 4 Stims
- Shipping Condition Dry Ice
- Storage Condition -20
- Pack Length (m) 0.015
- Pack Width (m) 0.015
- Pack Height (m) 0.05
- Pack Weight (kg) 0.10