This product is for Professional Use only and not for sale to the general public for home use.
Please log in with a business account to purchase.

Sign In

Total ketone bodies acetoacetate AcAc 3-hydroxybutyrate 3-HB enzymatic assay R1 3-1000µmol/L Standard 0.2-200µmol/L High-Sens

Product code: 415-73301

Pack Size: 2x27ml
CE Verified: CE IVD
Contact us

This is not a regular stock item and therefore not subject to our standard delivery schedule. Please contact us on +44 (0) 2380 483000 for advice on expected delivery date.

Product Information

Total Ketone Bodies Assay R1 manufactured by FUJIFILM Wako Pure Chemical Corporation

Glucose is the preferred energy source for cells when this is not available the body will switch to fat metabolism to produce energy. Ketones are a product of fat metabolism and levels of ketones will build up in the blood, firstly producing ketosis and then progressing to ketoacidosis. Ketoacidosis is a form of metabolic acidosis most often seen is individuals with uncontrolled type I diabetes, although it can occur in type II diabetes, during pregnancy, illness and severe infections. There are three ketone bodies - acetoacetate, acetone and beta-hydroxybutyrate (a.k.a. 3-HB). Beta-hydroxybutyrate (3-Hydroxybutyrate, 3-HB) is the main ketone present in severe diabetic ketoacidosis and measurement of 3-HB and Total Ketone bodies can be useful in the diagnosis and monitoring of diabetes. The AcAc/3-HB ratio in arterial blood reflects liver reserve capacity so may also be useful in the field of liver transplantation.

The Wako Total Ketone Bodies assay measures both acetoacetate (AcAc) and 3-Hydroxybutyrate (3-HB, also known as beta-hydroxybutyrate). When used in conjunction with the 3-HB assay, the concentration of AcAc can be calculated by subtracting the 3-HB value from the Total value. The Total Ketone Bodies assay is performed using the Total Ketone Bodies Reagent (415-77301) and the 3-HB Reagent 2 (413-73601). When the sample is mixed with these reagents a cyclic reaction is triggered whereby AcAc is reduced to give 3-HB (oxidation of NADH to NAD) and 3-HB is oxidised to give AcAc (reduction of Thio-NAD to Thio-NADH). Both AcAc and 3-HB are assayed by measuring the rate of Thio-NADH production at 405nm. Wako's Total Ketone Bodies assay can be used with serum or plasma (heparin, citrate oxalate, EDTA or sodium fluoride) samples and has a standard measuring range of 3-1000µmol/L (a high-sensitivity method using increased sample gives a range of 0.2-200µmol/L).

The assay utilises two reagents: Total Ketone Bodies Reagent 1 (415-73301) is prepared by reconstituting the lyophilised Thio-NAD with phosphate buffer, this solution is stable for up to 3 weeks when stored at 2-10C. 3-HB Reagent 2 (417-73501) is prepared by combining lyophilised 3-HBDH and NADH with Good's Buffer; the reconstituted reagent is stable for up to 3 weeks when stored at 2-10C. The Total Ketone Bodies assay should be calibrator with the Ketone Body Calibrator (412-73791). 3-HB can be determined by using 3-HB Reagent 1 (417-73501) with 3-HB Reagent 2 (417-73501) and AcAc calculated by subtracting 3-HB from Total Ketones.


  • Brand FUJIFILM Wako Chemicals Europe GmbH – Diagnostics
  • Sterile N
  • CE Certified CE IVD
  • Warranty Period Standard Terms Apply
  • Is product classified as dangerous goods? No
  • Does the product contain latex? Unspecified
  • CAS Number NULL
  • Composition lyophilised reagent and buffer for reconstitution
  • Concentration standard method 3-1000µmol/L high-sensitivity method 0.2-200µmol/L
  • Detection Method kinetic
  • Diagnostic Target serum plasma total ketone bodies
  • Form 2x27ml; 2 vials lyophilised reagent.
  • Marketed Grade IVD CE
  • Purity NULL

Storage Details

  • Pack Description 2x27ml
  • Shipping Condition Blue Ice
  • Storage Condition Fridge
  • Pack Length (m) 0.1
  • Pack Width (m) 0.1
  • Pack Height (m) 0.1
  • Pack Weight (kg) 1.00

Require further information for this product? Get in touch below

How can we contact you? Please confirm accepted methods:

* Required Fields

Your request will be forwarded to the relevant department to assist with your enquiry. By clicking 'Send Enquiry' you are authorising Alpha Laboratories to process your information and use it to contact you so that we may respond to your request, as well as to communicate with you regarding our products and services.

Submitting your details indicates that you agree to our Privacy Policy.