Testosterone ELISA for serum or plasma 0.10-16 ng/ml Dialab

Testosterone (17β-Hydroxy-4-androstene-3-one) is a steroid hormone from the androgen group mainly produced by the Leydig cells located in the testes and, minimally, by the ovaries and adrenal cortex. In women there is a greater tendency to convert this hormone into oestrogen, In postpubertal males, testosterone is secreted primarily by the testes with only a small amount derived from peripheral conversion of androstenedione. In humans testosterone stimulates the development of the sexual organs and of secondary sexual characteristics such as beard hair distribution, the timbre of the voice and muscles. During puberty, testosterone us also involved in skeletal development, limiting elongation of long bones and thereby avoiding a disproportionate growth of the limbs.
It helps to ensure male fertility, stimulating maturation of the sperm in the tested and influencing quality and quantity of the sperm produced. Daily testosterone production in males is 5-7mg but after 30 years of age this begins to decline. In adult women over 50% of serum testosterone is derived from peripheral conversion of androstenedione secreted by the adrenal gland and the ovary, the remainder is secreted directly by these glands. The majority of circulating testosterone is bound by sex-hormone-binding globulin (SHBG) and a smaller portion bound to albumin. Less than 1% or circulating hormone is free.
In females, elevated testosterone levels can be associated with a variety of virilising conditions, including adrenal tumours and polycystic ovarian disease.

This assay is intended for the quantitative determination of total testosterone in human serum or plasma for diagnostic use. This 96 well ELISA can provide up to 40 patient tests. Testosterone in the sample competes with horseradish peroxidase (HRP) labelled testosterone for binding onto the limited number of anti- testosterone sites coated on the microplate. After incubation, the bound/free separation is performed by a simple- solid-phase washing. The enzyme in the bound-fraction reacts with the substrate to develop a blue colour which changes to yellow on addition of the stop solution. The colour intensity, as determined by reading absorbance at 450nm, is inversely proportional to the concentration of testosterone in the sample.

Antibody coated Microtiter wells: 12x8 well strips Anti-Testosterone IgG
Calibrator set: 5x1ml (0, 0.2, 1.0, 4.0, 16.0 ng/ml)
Testosterone Control: 2x1ml
Enzyme Conjugate (Testosterone-HRP): 1x12ml
TMB Substrate Solution : 1x15ml
Stop Solution: 1x15ml
Wash Buffer 10X: 1x50ml
Sample volume: 25µl human serum or plasma to be run in duplicate
Incubation time: 75 minutes (60 min at 37°C,15 min at room temperature)
Calibration range: 0 – 16.0 ng/ml
Sensitivity: 0.10 ng/ml
Store at 2-8°C.