MagCapture Exosome Isolation Kit PS Ver2

Extracellular Vesicles（ EVs） represented by exosomes contain a protein, mRNA, microRNA, DNA, and lipids on the surface or inside. After being secreted from various cells, EVs stably exist in body fluids such as blood, urine, saliva, cerebrospinal fluid, and breast milk. Thus, it is attracting attention as a messenger for cell-to-cell communication and as a biomarker for diseases. As a method for isolating exosomes, an ultracentrifugation method, an affinity method using an antibody against a surface antigen, a precipitation method using a polymer reagent etc. are generally used. However, in all respects such as recovery efficiency, purity, and operability, there was no satisfying way. MagCaptureTM Exosome Isolation Kit PS Ver.2 adopts a novel affinity purification method using magnetic beads and phosphatidylserine (PS）-binding protein （PS affinity method）. The PS affinity method can easily isolate exosomes and other EVs with PS on the membrane surface from the cell culture medium and body fluids with high purity and high efficiency. Using the 10,000×g supernatant as a sample, PS affinity can get more highly purified exosomes. Besides, PS affinity enables the elution of intact EVs from magnetic beads with a chelating agent because PS affinity captures EVs in a metal ion-dependent manner. Therefore, purified EVs can be used for various applications such as electron microscopic analysis, nanoparticle tracking analysis, uptake assay, and analysis of constituent molecules（ proteins, lipids, nucleic acids, etc.).