QuickGene-Mini480 DNA RNA Extractor Instrument Semi-Automated Large for DNA and RNA Extraction

The QuickGene series of instruments and kits provides a novel, innovative method for DNA and RNA extraction from a vast number of sample types, all without the need for strong centrifugation steps, or centrifugation at all.

QuickGene operates in a similar manner to centrifugation methods, isolating DNA from samples by forcing said samples through a membrane. This membrane has a hydrophilic surface and therefore retains other hydrophilic molecules, such as DNA or RNA depending on the target, but does not retain hydrophobic molecules such as proteins. The result is that unwanted molecules are washed through the membrane, whereas DNA or RNA are retained in the membrane and eluted into collection tubes.

However, the major difference comes from the QuickGene porous membrane itself, which is 10 times thinner than conventional, usually glass fibre based, membranes. Traditional glass fibre membranes are roughly 1000µm in thickness and require large forces, created by centrifugation, to force samples through this thick membrane. QuickGene on the other hand, uses porous membranes only 100µm thick, requiring much weaker force to push samples through the membrane. This force is generated by compressed air, supplied by the QuickGene nucleic acid instruments, such as the QuickGene Mini480. The use of compressed air eliminates the need for centrifugation and allows weaker forces to be applied to the sample allowing for improved preservations, and therefore increased length, of nucleic acid chains, whilst the innovative membrane retains high nucleic acid purity and yield.

The QuickGene Mini480 nucleic acid extraction device is designed for the semi-automated extraction of nucleic acids from smaller samples of up to 200µL. The Mini480 can process up to 48 samples at a time, with operation as simple as turning the knob to turn the system on and off. Once processed, the isolated and purified DNA is suitable for use in many downstream analyses including, among others: sequencing, PCR, blotting, DNA micro arrays and digestion.

Through the use of the following corresponding QuickGene DNA extraction kits a wide variety of sample types can be processed, including:

•630-23549 DB-S DNA Whole Blood Kit S:

•Human Blood Samples
•Animal Blood Samples

•637-23559 DT-S DNA Tissue Kit S
•638-23609 RT-S2 RNA Tissue Kit SII:

•Brain
•Saliva
•Liver
•Lung
•Kidney
•Hard Tissues (Teeth and Bone)
•Hair
•Nails
•Plant Tissues
•Paraffin Embedded Samples

•634-23569 Plasmid PL-SII

•Bacteria
•Viruses


•638-23589 RNA Cultured Cell Kit S 96 Tests
•635-23599 QuickGene RNA cultured cell HC kit 96/48:

•Cultured Cells
•631-23579 RB-S RNA Blood Cell Kit S 96 Tests

The QuickGene Mini480 can also be adapted for use with larger samples and kits through the use of an adaptor, further expanding its capabilities.

Specifications

•Sample Throughput: 1-48 Samples per cycle
•Maximum Sample Volume: 200µL
•Width: 280mm
•Length: 260mm
•Height: 300mm
•Weight: 3.3kg
•Voltage: 100-240V
•Operating Temperature: 15-30C