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microRNA Isolation Kit Human Rodent serum samples precipitated miRNA Wako
Product code: 295-71701
Pack Size: 50 Tests
CE Verified: Research Use Only
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Product Information
microRNA Extractor SP Kit produced by FUJIFILM Wako Chemical Corporation
MicroRNAs (miRNAs) are small noncoding RNA sequences, typically around 22 nucleotides in length, which act as post-transcriptional regulators of gene expression. Mature miRNAs are localised in the cytoplasm where they bind and assemble into the RNA-induced silencing complex (RISC) to target messenger RNA (mRNA). At the heart of every RISC is a member of the Argonaute protein family, which serves to bind the miRNAs and position them in a way that facilitates target recognition. The miRNA bind complementary sequences in the target and the RISC may then directly cleave the target RNA or recruit other gene-silencing proteins to inhibit translation.
Circulating miRNA in blood and other body fluids may be associated with disease and have been proposed as potential biomarkers for cancer and other diseases. The microRNA Extractor SP Kit is designed for extraction of micro-RNA from serum or plasma without the use of phenol or chloroform. The microRNA fractions recovered are suitable for analysis by real-time quantitative PCR.
Kit Contents (50 Reactions):
Lysis Solution 19ml
Reducing Agent 50µl
Protease Solution 500µl
Enhancer 500µl
Wash Solution I 12ml
Wash Solution II 21ml
Elution Solution 5ml
Spin Column 50
Collection Tube 50
MicroRNAs (miRNAs) are small noncoding RNA sequences, typically around 22 nucleotides in length, which act as post-transcriptional regulators of gene expression. Mature miRNAs are localised in the cytoplasm where they bind and assemble into the RNA-induced silencing complex (RISC) to target messenger RNA (mRNA). At the heart of every RISC is a member of the Argonaute protein family, which serves to bind the miRNAs and position them in a way that facilitates target recognition. The miRNA bind complementary sequences in the target and the RISC may then directly cleave the target RNA or recruit other gene-silencing proteins to inhibit translation.
Circulating miRNA in blood and other body fluids may be associated with disease and have been proposed as potential biomarkers for cancer and other diseases. The microRNA Extractor SP Kit is designed for extraction of micro-RNA from serum or plasma without the use of phenol or chloroform. The microRNA fractions recovered are suitable for analysis by real-time quantitative PCR.
Kit Contents (50 Reactions):
Lysis Solution 19ml
Reducing Agent 50µl
Protease Solution 500µl
Enhancer 500µl
Wash Solution I 12ml
Wash Solution II 21ml
Elution Solution 5ml
Spin Column 50
Collection Tube 50
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Efficiency NULL
- Shelf Life NULL
- Yield NULL
- Purification Method Spin-Column solid-phase extraction
- Purity NULL
- Application Isolation of microRNA in human or rodent serum or plasma
- Contents Lysis Solution, Reducing Agent, Protease Solution, Enhancer, Wash Solutions, Elution Solution, Spin Columns and Collection Tubes
- Type miRNA
- Time Required NULL
- Starting Material Human/Rodent Serum or plasma
Storage Details
- Pack Description 50 Tests
- Shipping Condition Direct
- Storage Condition Special
- Pack Length (m) 0.2
- Pack Width (m) 0.16
- Pack Height (m) 0.12
- Pack Weight (kg) 1.50