Isoelectric focussing system (IEF) for first dimension IEF with IPG (immobilised pH gradient) strips

Now equipped with rehydration and focusing trays, the redesigned Clarit-E IEF gel tank has been optimised to perform first-dimension isoelectric focusing (IEF) with IPG (immobilised pH gradient) strips quickly, easily and reproducibly. An ideal entry-level system for both inexperienced and occasional IEF users, the Clarit-E IEF gel tank is also versatile enough to meet the needs of laboratories with increased throughput requirements.
Features include:
• A high-capacity focusing tray that accommodates up to twelve IPG strips
• Adjustable ‘pick-and-place’ electrodes clip conveniently anywhere within the focusing tray to resolve IPG strips 7-24cm in length; colour-coded to prevent polarity reversal
• A cooling plate, manufactured from a special grade ceramic in a large 26x26cm surface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high voltage IEF techniques
• An optional, but recommended, recirculating chiller (EL7010) connects quickly and easily to the cooling plate via snap-lock connectors to maintain optimal operating temperatures for IPG strips (20°C) and precast gels (4°C)
• Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF to maximise throughput, and provides useful storage at -20°C for focused strips before second-dimension runs
• Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels

TYPICAL RUNNING CONDITIONS
7cm IPG Strip
IEF Step: 1 2 3 4 5 6
Voltage (V): 150 300 600 1500 3000 300
Time (h): 0.5 0.5 0.5 0.5 2.5 < 20
Volt-hours: 75 150 300 750 7500 -
18cm IPG Strip
IEF Step: 1 2 3 4 5
Voltage (V): 300 600 1500 3000 300
Time (h): 0.5 1 1 12 < 20
Volt-hours: 150 600 1500 36000 -
TRAY SPECIFICATIONS
IPG Strip Length: 7cm 11cm* 18cm 24cm
FOCUSING TRAY
Electrode Distance: 6.5cm 10.2cm 17.1cm 22.7cm
Maximum Strip Length Accommodated: 25.3cm 25.3cm 25.3cm 25.3cm
Cleaver Scientific IPG Strip Length: 7cm n/a* 18cm 24cm
REHYDRATION TRAY
Maximum Strip Length Accommodated: 24cm 24cm 24cm 24cm
Recommended Volume for Strip Rehydration: 3.5ml 6ml 8ml 12ml
*11cm strips available from other suppliers
SPECIFICATIONS
Max. commercial strip length accommodated: 24cm
Max. gel dimensions on cooling plate: 26x26cm
Unit dimensions (w x d x h): 55x35x10cm
Focusing tray strip capacity: 12x 18 and 24cm strips; 24x 7 and 11cm strips
Operating temperature: 4-25°C
Regulatory certification: CE, EN61010