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Endoproteinase Asp N neutral protease sequencing grade Wako
Product code: 056-05921
Pack Size: 2µg
CE Verified: Research Use Only
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Product Information
Endoproteinase Asp N produced by FUJIFILM Wako Chemical Corporation
Sequencing grade endopeptidase Asp-N cleaves polypeptide chains under mild conditions where modifications such as protease mediated oxidation, halogenation, deamidation, etc. of side-chain amino acid residues do not occur.
Peptides containing groups (binding sites in carbohydrate chains, lipids, phosphate, sulphuric acid, etc.) for modification are usually recovered in a complete form at a good yield. Segments with good reproducibility can be obtained under identical conditions of digestion. Highly substrate-specific enzymes are used for the primary structure analysis of proteins, because it is possible to roughly predict the number of fragments and their average sizes from amino-acid composition, and more importantly, because it is possible to avoid complex isolation due to partial (nonspecific) cleavages.
This enzyme specifically cleaves the N-terminal end of aspartic acid residue in proteins and peptides. The solution after preparation should be stored -20 degrees centigrade.
Specificity: Reaction rate after 1 hour using glucagon as a substrate: min 90%
Source: Pseudomonas fragi mutant
Min 20,000U/mg
CAS: 9001-92-7
Store 2-10C.
Sequencing grade endopeptidase Asp-N cleaves polypeptide chains under mild conditions where modifications such as protease mediated oxidation, halogenation, deamidation, etc. of side-chain amino acid residues do not occur.
Peptides containing groups (binding sites in carbohydrate chains, lipids, phosphate, sulphuric acid, etc.) for modification are usually recovered in a complete form at a good yield. Segments with good reproducibility can be obtained under identical conditions of digestion. Highly substrate-specific enzymes are used for the primary structure analysis of proteins, because it is possible to roughly predict the number of fragments and their average sizes from amino-acid composition, and more importantly, because it is possible to avoid complex isolation due to partial (nonspecific) cleavages.
This enzyme specifically cleaves the N-terminal end of aspartic acid residue in proteins and peptides. The solution after preparation should be stored -20 degrees centigrade.
Specificity: Reaction rate after 1 hour using glucagon as a substrate: min 90%
Source: Pseudomonas fragi mutant
Min 20,000U/mg
CAS: 9001-92-7
Store 2-10C.
Specification
- Brand FUJIFILM Wako Chemicals Europe GmbH – Lab Chem
- Sterile N
- CE Certified Research Use Only
- Warranty Period Standard Terms Apply
- Is product classified as dangerous goods? No
- Does the product contain latex? Unspecified
- Specific Activity NULL
- Purity NULL
- Concentration Min 20,000U/mg
- EC Number NULL
- Optimal Temperature NULL
- Optimal pH NULL
- Source Species Pseudomonas fragi mutant
- Application Specifically cleaves the N-terminal end of aspartic acid residue in proteins and peptides
- CAS Number 9001-92-7
- Unit Definition NULL
Storage Details
- Pack Description 2µg
- Shipping Condition Blue Ice
- Storage Condition Fridge
- Pack Length (m) 0.04
- Pack Width (m) 0.04
- Pack Height (m) 0.08
- Pack Weight (kg) 1.00